A high-affinity nitrate transporter PaNRT2.1 mediates dark septate endophyte (DSE) dependent nitrogen assimilation in sweet cherry roots
文献类型: 外文期刊
第一作者: Wu, Fanlin
作者: Wu, Fanlin;Qu, Dehui;Zhao, Xiaohui;Su, Hongyan;Zhu, Dongzi;Zhang, Xu;Wang, Lei
作者机构:
关键词: DSE; PaNRT2; 1; Sweet cherry; Symbiosis; PaCRK10
期刊名称:PLANT AND SOIL ( 影响因子:4.9; 五年影响因子:5.2 )
ISSN: 0032-079X
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Background and aimsLow nitrogen (N) soil environment is one of the main factors limiting crop yield and horticultural plant growth. Dark septate endophytes (DSEs) are co-occurring in 80% of terrestrial plants and they can promote the absorption of N. However, few data on the contribution of DSEs symbiosis to plant N acquisition and their molecular mechanisms are available.MethodsThe growth, N contents and expression of N related genes of Gisela 5, a sweet cherry rootstock colonized with and without DSE fungus S16, were measured under low and normal nitrate conditions. Subsequently, molecular identification and functional study of PaNRT2.1, a nitrate transporter highly responsive to S16, were conducted.ResultsIn this study, we found that S16 promoted the growth and N uptake of Gisela 5 seedlings under low and normal nitrate conditions, and induced the expression of the nitrate transporter gene PaNRT2.1 in roots. The expression of PaNRT2.1 significantly affected the nitrate uptake of Gisela 5 roots and the symbiosis with S16 under low nitrate conditions. Transcriptome analysis showed that a receptor-like kinase gene PaCRK10 was significantly induced during S16 symbiosis and PaNRT2.1 overexpression, indicating a signal transduction between DSE colonization with nitrate uptake. Further studies showed that there was a strong interaction between PaNRT2.1 and PaCRK10.ConclusionsTaken together, our results provide a deeper understanding of how DSE promoted N uptake by sweet cherry rootstock Gisela 5 and a more promising approach for improving N uptake efficiency through plant-microbe interactions.
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