Pold4 is dispensable for mouse development, DNA replication and DNA repair
文献类型: 外文期刊
第一作者: Gu, Xueping
作者: Gu, Xueping;Dai, Qinjin;Du, Peng;Li, Ning;Li, Jiahui;Zeng, Simiao;Peng, Shuyi;Tang, Shengjun;Zhou, Zhongcheng;Wang, Lei
作者机构:
关键词: Pold4; DNA replication; DNA damage; DNA repair; CRISPR
期刊名称:GENE ( 影响因子:3.5; 五年影响因子:3.3 )
ISSN: 0378-1119
年卷期: 2023 年 851 卷
页码:
收录情况: SCI
摘要: The DNA polymerase delta (Pol delta), a heterotetramer of four subunits (Pol delta 4), plays a pivotal role in DNA replication, as well as in DNA damage repair. Pold4, as the smallest subunit of Pol delta, is degraded in response to DNA damage or when entering into S-phase. This leads to the conversion of Pol delta 4 to the trimeric complex Pol delta 3. However, the contribution of Pold4 has not been fully elucidated in mammals. Cdm1, the Pold4 ortholog in Schizosaccharomyces pombe, is dispensable for cell growth and DNA damage repair, and there are no Pold4 orthologs in Saccharomyces cerevisiae. We previously generated a knockout mouse model of Pold3 and revealed its essential role in genome stability. Unexpectedly, we here found that Pold4 knockout mice are viable and fertile. In addition, Pold4 knockout mice do not exhibit any pathologic changes in the lung and spleen, tissues with the most abundant expression of Pold4. Moreover, Pold4 knockout mouse tail tip fibroblasts (TTF) exhibited normal cell growth, cell cycle, DNA replication, DNA damage and DNA repair capacity. These results suggested that Pol delta 3 but not Pol delta 4 may be responsible for these processes in normal cells. Interestingly, 19-month-old wild-type (WT) mice had tumors in the liver, while Pold4 knockout mice did not, and Pold4 knockout mice showed increased longevity. In further, this provided evidence suggested that Pold4 could be a potential novel target for lung carcinoma because its depletion does not affect normal cells but does affect cancer cells.
分类号:
- 相关文献
作者其他论文 更多>>
-
A slow-release fertilizer containing cyhalofop-butyl reduces N2O emissions by slowly releasing nitrogen and down-regulating the relative abundance of nirK
作者:Zhang, Zewang;Yu, Xiangyang;Liang, Dong;Xu, Cong;Ji, Cheng;Wang, Lei;Ma, Hongbo;Wang, Jidong;Zhang, Zewang;Wang, Jidong;Chen, Gonglei
关键词:Slow-release fertilizer; Cyhalofop-butyl; Nitrous oxide; Bacteria; Nitrogen functional genes
-
Urate oxidase from tea microbe Colletotrichum camelliae is involved in the caffeine metabolism pathway and plays a role in fungal virulence
作者:He, Shengnan;Zhang, Shuhan;Xia, Jinglin;Liu, Shouan;Qiao, Xiaoyan;Wang, Lei;Liu, Shouan
关键词:Colletotrichum camelliae; tea plant; caffeine metabolism; urate oxidase; purine alkaloids
-
Engineering Cupriavidus necator H16 for heterotrophic and autotrophic production of myo-inositol
作者:Wang, Xiaolu;Wang, Kaikai;Wang, Lei;Luo, Huiying;Wang, Yaru;Wang, Yuan;Tu, Tao;Qin, Xing;Su, Xiaoyun;Bai, Yingguo;Yao, Bin;Huang, Huoqing;Zhang, Jie;Wang, Lei
关键词:Cupriavidus necator; Metabolic engineering; Myo-inositol; Renewable feedstocks; CO2
-
Plow layer management during the fallow season can enhance the wheat productivity and resource utilization in a semi-arid region
作者:Zhao, Gang;Li, Shangzhong;Zhang, Jianjun;Dang, Yi;Wang, Lei;Wang, Shuying;Cheng, Wanli;Fan, Tinglu;Wu, Peng;Wu, Peng;Liu, Fu;Cai, Tie
关键词:Green manure; Precipitation storage efficiency; Soil water; Soil quality; Wheat productivity
-
Long-term partial substitution of chemical fertilizer by organic amendments influences soil microbial functional diversity of phosphorus cycling and improves phosphorus availability in greenhouse vegetable production
作者:Zhang, Yinjie;Ma, Long;Tang, Jiwei;Huang, Shaowen;Wang, Lei;Gao, Wei;Li, Mingyue;Luan, Haoan;Li, Ruonan
关键词:Soil phosphorus cycling; Metagenome; gcd gene; P solubilization; Genome
-
Long-term anthropogenic field management enhanced wheat productivity and resource utilization in the Loess Plateau region of China
作者:Wu, Peng;Wu, Qi;Huang, Hua;Xue, Jianfu;Sun, Min;Gao, Zhiqiang;Zhao, Gang;Li, Shangzhong;Wang, Lei;Cheng, Wanli;Fan, Tinglu;Zhang, Peng;Fu, Jian;Yang, Kejun;Bangura, Kemoh;Gao, Zhiqiang
关键词:Organic fertilizer; Soil fertility; Straw incorporation; Water and nitrogen utilization; Wheat productivity
-
Revisiting Horseradish Peroxidase-Catalyzed Aerobic Oxidation of NAD(P)H in the Presence of Mn(II) and Mediators: Application in Catalytic Oxidation
作者:Dai, Jian-Rong;Sha, Jiang-Tao;Zong, Min-Hua;Li, Ning;Wang, Zhi-Lin
关键词:chemobiocatalysis; cofactor regeneration; dehydrogenases; multienzyme catalysis; oxidation