Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant smk7a in Different Development Stages

文献类型： 外文期刊

第一作者： Wang, Jing

作者： Wang, Jing;Wang, Hongwu;Peng, Yunling;Wang, Jing;Wang, Hongwu;Li, Kun;Liu, Xiaogang;Cao, Xiaoxiong;Zhou, Yuqiang;Huang, Changling;Hu, Xiaojiao;Wang, Jing;Wang, Hongwu;Peng, Yunling

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关键词： maize; smk7a mutant; IAA; starch synthesis; transcriptome

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 2 期

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收录情况： SCI

摘要： The kernel serves as a storage organ for various nutrients and determines the yield and quality of maize. Understanding the mechanisms regulating kernel development is important for maize production. In this study, a small-kernel mutant smk7a of maize was characterized. Cytological observation suggested that the development of the endosperm and embryo was arrested in smk7a in the early development stage. Biochemical tests revealed that the starch, zein protein, and indole-3-acetic acid (IAA) contents were significantly lower in smk7a compared with wild-type (WT). Consistent with the defective development phenotype, transcriptome analysis of the kernels 12 and 20 days after pollination (DAP) revealed that the starch, zein, and auxin biosynthesis-related genes were dramatically downregulated in smk7a. Genetic mapping indicated that the mutant was controlled by a recessive gene located on chromosome 2. Our results suggest that disrupted nutrition accumulation and auxin synthesis cause the defective endosperm and embryo development of smk7a.

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