PCSK9-D374Y Suppresses Hepatocyte Migration through Downregulating Free Cholesterol Efflux Rate and Activity of Extracellular Signal-Regulated Kinase
文献类型: 外文期刊
第一作者: Huang, Lei
作者: Huang, Lei;Cheng, Ying;Li, Kui;Mu, Yulian
作者机构:
期刊名称:ANALYTICAL CELLULAR PATHOLOGY ( 影响因子:3.2; 五年影响因子:3.6 )
ISSN: 2210-7177
年卷期: 2023 年 2023 卷
页码:
收录情况: SCI
摘要: Proprotein convertase subtilisin/kexin type 9 can mediate the intracellular lysosomal degradation of the low-density lipoprotein receptor protein in hepatocytes and decrease the liver's ability to scavenge low-density lipoprotein cholesterol from circulation, resulting in high levels of cholesterol in the circulatory system. Current studies have primarily focused on the relationship between PCSK9 and blood lipid metabolism; however, the biological function of PCSK9 in hepatocytes is rarely addressed. In this study, we evaluate its effects in the human hepatoma carcinoma cell line HepG2, including proliferation, migration, and free cholesterol transport. PCSK9-D374Y is a gain-of-function mutation that does not affect proliferation but significantly suppresses the migration and cholesterol efflux capacity of these cells. The suppression of the transmembrane outflow of intracellular-free cholesterol regulates small G proteins and the suppression of extracellular signal-regulated kinase. In summary, PCSK9-D374Y affects hepatocyte features, including their migration and free cholesterol transport capabilities.
分类号:
- 相关文献
作者其他论文 更多>>
-
Simultaneous change of microworld and biofilm formation in constructed wetlands filled with biochar
作者:Zhong, Hui;Jiang, Chunli;He, Xi;He, Jinke;Zhao, Yaqi;Chen, Yucheng;Huang, Lei;Zhong, Hui;Jiang, Chunli;He, Xi;He, Jinke;Zhao, Yaqi;Chen, Yucheng;Huang, Lei;Huang, Lei;Huang, Lei
关键词:Constructed wetland; Biochar; Biofilm; Extracellular polymeric substance; Extracellular protein
-
Single-cell omics: A new direction for functional genetic research in human diseases and animal models
作者:Kong, Siyuan;Li, Rongrong;Tian, Yunhan;Zhang, Yaqiu;Lu, Yuhui;Ou, Qiaoer;Gao, Peiwen;Li, Kui;Zhang, Yubo;Kong, Siyuan;Tian, Yunhan;Li, Kui;Zhang, Yubo;Zhang, Yubo
关键词:single-cell omics; multi-omics integration; human disease; cell lines; animal disease models
-
Characterization of Gut Microbiota Compositions along the Intestinal Tract in CD163/pAPN Double Knockout Piglets and Their Potential Roles in Iron Absorption
作者:Zhang, Xiu-Ling;Xu, Kui;Xu, Chang-Jiang;Che, Jing-Jing;Liu, Zhi-Guo;Wang, Bing-Yuan;Mu, Yu-Lian;Li, Kui;Zhang, Xiu-Ling;Zhou, Yan-Rong;Xu, Si;Xiong, Yu-Jian;Xiao, Shao-Bo;Zhou, Yan-Rong;Xu, Si;Xiong, Yu-Jian;Xiao, Shao-Bo;Xu, Song-Song;Huang, Lei;Li, Kui
关键词:16S rRNA; iron absorption; gut microbiota; piglet; carboxylic acids
-
Pathways and biological mechanisms of N2O emission reduction by adding biochar in the constructed wetland based on 15N stable isotope tracing
作者:Huang, Lei;Xiong, Haifeng;Jiang, Chunli;He, Jinke;Lyu, Wanlin;Chen, Yucheng;Huang, Lei;Xiong, Haifeng;Jiang, Chunli;He, Jinke;Lyu, Wanlin;Chen, Yucheng;Huang, Lei;Chen, Yucheng;Huang, Lei
关键词:Constructed wetland; Biochar; Nitrous oxide; Emission pathway; Functional microbes; Enzyme activity
-
PRRSV alters m6A methylation and alternative splicing to regulate immune, extracellular matrix-associated function
作者:Lin, Chenghong;Zeng, Mu;Song, Jia;Li, Hua;Feng, Zheng;Pei, Yangli;Li, Kui
关键词:Alternative splicing; N6-methyladenosine; PRRSV
-
OONO-/MMP2/MMP9 pathway-mediated apoptosis of porcine granulosa cells is associated with DNA damage
作者:Lei, Kun;Wei, Quanwei;Cheng, Ying;Wang, Zhe;Wu, Haoze;Shi, Fangxiong;Zhao, Fang;Ding, Wei
关键词:
-
Strategies to alleviate clogging in constructed wetlands: What can be learned from the microbial fuel cell coupled membrane bioreactor?
作者:Huang, Lei;He, Jinke;Jiang, Chunli;Weng, Sijia;Zhao, Fang;Zhong, Hui;Chen, Yucheng;Huang, Lei;He, Jinke;Jiang, Chunli;Weng, Sijia;Zhao, Fang;Zhong, Hui;Chen, Yucheng;Huang, Lei;Chen, Yucheng;Chen, Yucheng
关键词:Constructed wetland; Bioelectrochemistry; Microbial fuel cell; Biological clogging; Extracellular polymeric substances