PRRSV alters m6A methylation and alternative splicing to regulate immune, extracellular matrix-associated function
文献类型: 外文期刊
第一作者: Lin, Chenghong
作者: Lin, Chenghong;Zeng, Mu;Song, Jia;Li, Hua;Feng, Zheng;Pei, Yangli;Li, Kui
作者机构:
关键词: Alternative splicing; N6-methyladenosine; PRRSV
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:8.2; 五年影响因子:7.8 )
ISSN: 0141-8130
年卷期: 2023 年 253 卷
页码:
收录情况: SCI
摘要: The alternative splicing and N6-methyladenosine (m6A) modifications occurring during porcine reproductive and respiratory syndrome virus (PRRSV) infections remain poorly understood. Transcriptome and MeRIP-seq analyses were performed to identify the gene expression changes, splicing and m6A modifications in the lungs of PRRSV-infected pigs. In total, 1624 differentially expressed genes (DEGs) were observed between PRRSVinfected and uninfected pigs. We observed significant alterations in alternative splicing (54,367 events) and m6A modifications (2265 DASEs) in numerous genes, including LMO7, SLC25A27, ZNF185, and ECM1, during PRRSV infection. LMO7 and ZNF185 exhibited alternative splicing variants and reduced mRNA expression levels following PRRSV infection. Notably, LMO7 inhibited c-JUN, SMAD3, and FAK expression, whereas ZNF185 affected the expression of FAK, CDH1, and GSK3 beta downstream. Additionally, ECM1 influenced FAK expression by targeting ITGB3 and AKT2, suggesting its involvement in extracellular matrix accumulation through the ITGB3-AKT2/FAK pathway. These changes may facilitate viral invasion and replication by modulating the expression of genes and proteins participating in crucial cellular processes associated with immunity and the extracellular matrix. We highlight the importance of these genes and their associated pathways in PRRSV infections and suggest that targeting these may be a promising therapeutic approach for treating viral infections.
分类号:
- 相关文献
作者其他论文 更多>>
-
Single-cell omics: A new direction for functional genetic research in human diseases and animal models
作者:Kong, Siyuan;Li, Rongrong;Tian, Yunhan;Zhang, Yaqiu;Lu, Yuhui;Ou, Qiaoer;Gao, Peiwen;Li, Kui;Zhang, Yubo;Kong, Siyuan;Tian, Yunhan;Li, Kui;Zhang, Yubo;Zhang, Yubo
关键词:single-cell omics; multi-omics integration; human disease; cell lines; animal disease models
-
Characterization of Gut Microbiota Compositions along the Intestinal Tract in CD163/pAPN Double Knockout Piglets and Their Potential Roles in Iron Absorption
作者:Zhang, Xiu-Ling;Xu, Kui;Xu, Chang-Jiang;Che, Jing-Jing;Liu, Zhi-Guo;Wang, Bing-Yuan;Mu, Yu-Lian;Li, Kui;Zhang, Xiu-Ling;Zhou, Yan-Rong;Xu, Si;Xiong, Yu-Jian;Xiao, Shao-Bo;Zhou, Yan-Rong;Xu, Si;Xiong, Yu-Jian;Xiao, Shao-Bo;Xu, Song-Song;Huang, Lei;Li, Kui
关键词:16S rRNA; iron absorption; gut microbiota; piglet; carboxylic acids
-
Alleviation of aqueous nitrogen loss from paddy fields by growth and decomposition of duckweed (Lemna minor L.) after fertilization
作者:Wang, Yuan;Chen, Xiaodong;Guo, Bin;Liu, Chen;Liu, Junli;Qiu, Gaoyang;Fu, Qinglin;Li, Hua
关键词:Duckweed; Nitrogen; Rice; Floodwater; Runoff loss
-
Toxicological response of Pacific white shrimp Litopenaeus vannamei to a hazardous cyanotoxin nodularin exposure
作者:Duan, Yafei;Xing, Yifu;Huang, Jianhua;Nan, Yuxiu;Li, Hua;Dong, Hongbiao;Duan, Yafei;Duan, Yafei
关键词:Shrimp; Cyanotoxins; Oxidative stress; Intestinal microbiota; Gene transcription; Metabolites
-
Alkaline humic acid fertilizer alters the distribution, availability, and translocation of cadmium and zinc in the acidic soil-Sauropus androgynus system
作者:Fan, Qiong;Jiu, Yuanda;Zou, Dongmei;Feng, Jian;Zhao, Min;Zhang, Qun;Lv, Daizhu;Song, Jia;Xu, Zhi;Ye, Haihui;Fan, Qiong;Jiu, Yuanda;Zou, Dongmei;Feng, Jian;Zhao, Min;Zhang, Qun;Lv, Daizhu;Song, Jia;Xu, Zhi;Ye, Haihui;Fan, Qiong;Jiu, Yuanda;Zou, Dongmei;Feng, Jian;Zhao, Min;Zhang, Qun;Lv, Daizhu;Song, Jia;Xu, Zhi;Ye, Haihui
关键词:Sauropus androgynus; Alkaline humic acid fertilizer; Heavy metal accumulation; Heavy metal translocation
-
Impact of ryegrass cover crop inclusion on soil phosphorus and pqqC- and phoD-harboring bacterial communities
作者:Chen, Xiaodong;Wang, Yuan;Wang, Jianghong;Guo, Bin;Liu, Junli;Qiu, Gaoyang;Li, Hua;Wang, Yuan;Qiu, Gaoyang;Condron, Leo M.;Li, Hua
关键词:Ryegrass-rice rotation; Soil phosphorus fractions; Phosphatase enzyme activities; Inorganic P-solubilizing and organic P; mineralizing bacteria; pqqC and phoD gene communities
-
Improvement of polyamine synthesis maintains photosynthetic function in wheat during drought stress and rewatering at the grain filling stage
作者:Zhao, Pengfei;Chen, Xinyi;Xue, Xinyu;Wang, Yuexia;Li, Hua;Xue, Ruili;Wang, Yongxia;Li, Yan;Wang, Yongxia;Li, Yan
关键词:Wheat cultivar; Drought tolerance; Polyamines; Photosynthesis; Regulation of gene expression