Overexpression of Sly-miR164a in tomato decreases expression of NAC and delays pre- and postharvest ripening of fruit
文献类型： 外文期刊
第一作者： Wang, Zhiqiang
作者： Wang, Zhiqiang;Duan, Wenhui;Meng, Lanhuan;Song, Hongmiao;Xu, Xiangbin;Wang, Qing;Li, Jiangkuo;Xu, Xiangbin
作者机构：
关键词： miR164; Tomato; Ripening; Postharvest; NAC
期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.751； 五年影响因子：7.001 ）
ISSN： 0925-5214
年卷期： 2023 年 195 卷
页码：
收录情况： SCI
摘要： Fruit ripening of tomato is regulated by NAC (NAM, ATAF1/2, and CUC2) domain proteins such as NOR-like1, SlNAC1, and SlNAC4. Acts as an up-stream regulator of NAC genes, the high abundance of miR164 might eliminate the impact of NAC on the ripening of fleshy fruit. In the present study, the STTM-miR164a (silencing miR164a by short tandem target mimic) and OE-miR164a (overexpression of miR164a) tomato lines were generated. Compared with those of silenced miR164a, overexpression of miR164a decreased ethylene production and lycopene accumulation, and delayed pre-and postharvest ripening of fruit. The expression analysis of target genes of miR164a revealed that the NAM3 (Solyc06g069710), GOB (Solyc07g062840) and NAC1 (Solyc07g066330) were involved in regulating fruit ripening.
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