Rapid and Visual Detection of SARS-CoV-2 RNA Based on Reverse Transcription-Recombinase Polymerase Amplification with Closed Vertical Flow Visualization Strip Assay
文献类型: 外文期刊
第一作者: Song, Yumeng
作者: Song, Yumeng;Huang, Pei;Yu, Mengtao;Jin, Hongli;Qiu, Jiazhang;Li, Yuanyuan;Zhang, Haili;Wang, Hualei;Li, Yuanguo;Gao, Yuwei
作者机构:
关键词: RT-RPA; SARS-CoV-2; amplification; detection; viral RNA; visual
期刊名称:MICROBIOLOGY SPECTRUM ( 影响因子:3.7; 五年影响因子:5.9 )
ISSN: 2165-0497
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was initially identified in 2019, after which it spread rapidly throughout the world. With the progression of the epidemic, new variants of SARS-CoV-2 with faster transmission speeds and higher infectivity have constantly emerged. The proportions of people asymptomatically infected or reinfected after vaccination have increased correspondingly, making the prevention and control of COVID-19 extremely difficult. There is therefore an urgent need for rapid, convenient, and inexpensive detection methods. In this paper, we established a nucleic acid visualization assay targeting the SARS-CoV-2 nucleoprotein (N) gene by combining reverse transcription-recombinase polymerase amplification with closed vertical flow visualization strip (RT-RPA-VF). This method had high sensitivity, comparable to that of reverse transcription-quantitative PCR (RT-qPCR), and the concordance between RT-RPA-VF and RT-qPCR methods was 100%. This detection method is highly specific and is not compatible with bat coronavirus HKU4, human coronaviruses 229E, OC43, and HKU1-CoV, Middle East respiratory syndrome coronavirus (MERS-CoV), or other respiratory pathogens. However, multiple SARS-CoV-2 variants are detectable within 25 min at 42 degrees C using this visual method, including RNA transcripts of the Wuhan-Hu-1 strain at levels as low as 1 copy/mu L, the Delta strain at 1 copy/mu L, and the Omicron strain at 0.77 copies/mu L. The RT-RPA-VF method is a simple operation for the rapid diagnosis of COVID-19 that is safe and free from aerosol contamination and could be an affordable and attractive choice for governments seeking to promote their emergency preparedness and better their responses to the continuing COVID-19 epidemic. In addition, this method also has great potential for early monitoring and warning of the epidemic situation at on-site-nursing points.IMPORTANCE The global COVID-19 epidemic, ongoing since the initial outbreak in 2019, has caused panic and huge economic losses worldwide. Due to the continuous emergence of new variants, COVID-19 has been responsible for a higher proportion of asymptomatic patients than the previously identified SARS and MERS, which makes early diagnosis and prevention more difficult. In this manuscript, we describe a rapid, sensitive, and specific detection tool, RT-RPA-VF. This tool provides a new alternative for the detection of SARS-CoV-2 variants in a range as low as 1 to 0.77 copies/mu L RNA transcripts. RT-RPA-VF has great potential to ease the pressure of medical diagnosis and the accurate identification of patients with suspected COVID-19 at point-of-care. The global COVID-19 epidemic, ongoing since the initial outbreak in 2019, has caused panic and huge economic losses worldwide. Due to the continuous emergence of new variants, COVID-19 has been responsible for a higher proportion of asymptomatic patients than the previously identified SARS and MERS, which makes early diagnosis and prevention more difficult.
分类号:
- 相关文献
作者其他论文 更多>>
-
A rabies virus-vectored vaccine expressing two copies of the Marburg virus glycoprotein gene induced neutralizing antibodies against Marburg virus in humanized mice
作者:Bi, Jinhao;Xia, Xianzhu;Bi, Jinhao;Wang, Haojie;Han, Qiuxue;Pei, Hongyan;Jin, Song;Chi, Hang;Yang, Songtao;Zhao, Yongkun;Yan, Feihu;Xia, Xianzhu;Han, Qiuxue;Xia, Xianzhu;Han, Qiuxue;Xia, Xianzhu;Pei, Hongyan;Wang, Hualei;Jin, Song;Ge, Liangpeng
关键词:Marburg virus disease; Marburg virus; neutralizing antibodies; fully humanized antibody; transgenic mice; CAMouse; MARV vaccine
-
Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge
作者:Sun, Si;Zuo, Qianfei;Xu, Chuanfei;Ou, Yangxue;Liu, Chang;Li, Haibo;Li, Chao;Lu, Dongshui;Zhang, Weijun;Luo, Ping;Cheng, Ping;Zou, Quanming;Guan, Shan;Gao, Yuwei;Tu, Changchun;Liu, Yan;Zhao, Gan;Sui, Cheng;Ding, Yuan;Wang, Bin;Tang, Jie;Cai, Larry;Pitard, Bruno;Rosenecker, Joseph;Wang, Bin;Wang, Bin;Liu, Yan;Zou, Quanming;Guan, Shan
关键词:Mucosal vaccine; SARS-CoV-2; DNA vaccine; Pulmonary delivery; Nonviral delivery system
-
Anti-inflammatory effects of theaflavin-3?-gallate during influenza virus infection through regulating the TLR4/MAPK/p38 pathway
作者:Sima, Mingwei;Deng, Xiuwen;Yue, Donghui;Gao, Yuwei;Sima, Mingwei;Lv, Chaoxiang;Qi, Jing;Guo, Jin;Luo, Rongbo;Deng, Xiuwen;Li, Yuanguo;Wang, Tiecheng;Gao, Yuwei;Qi, Jing;Guo, Jin;Yue, Donghui;Gao, Yuwei
关键词:Theaflavin derivatives; Influenza a virus; TLR4; MAPK; p38 signaling pathway; Anti-inflammatory
-
Schisandra chinensis (Turcz.) Baill. polysaccharide inhibits influenza A virus in vitro and in vivo
作者:Qi, Jing;Zhou, Yifa;Qi, Jing;Lv, Chaoxiang;Guo, Jin;Li, Yuanguo;Sima, Mingwei;Luo, Rongbo;Xiang, Haiyang;Xia, Xianzhu;Wang, Tiecheng;Lv, Chaoxiang;Guo, Jin;Sima, Mingwei;Wang, Tiecheng;Zhou, Yifa
关键词:antiviral; immunomodulation; inflammation; influenza; polysaccharides
-
Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (Camellia sinensis)
作者:Li, Yuanyuan;Zhang, Chenyu;Ma, Chunlei;Chen, Liang;Yao, Mingzhe
关键词:amino acid; bud mutation; Camellia sinensis; chlorophyll biosynthesis; flavonoid; RNA-seq
-
A potential Chinese medicine monomer against influenza A virus and influenza B virus: isoquercitrin
作者:Luo, Rongbo;Wang, Tiecheng;Deng, Xiuwen;Sima, Mingwei;Guo, Jin;Qi, Jing;Sun, Weiyang;Shen, Beilei;Li, Yuanguo;Gao, Yuwei;Lv, Chaoxiang;Wang, Tiecheng;Sun, Weiyang;Gao, Yuwei;Deng, Xiuwen;Sima, Mingwei;Gao, Yuwei;Guo, Jin;Gao, Yuwei;Qi, Jing;Yue, Donghui
关键词:Influenza A virus; Influenza B virus; Chinese medicine monomer; Isoquercitrin; Cytokines; NF-kappa B signaling; Method of drug administration
-
A key mutation in magnesium chelatase I subunit leads to a chlorophyll-deficient mutant of tea (Camellia sinensis)
作者:Zhang, Chenyu;Liu, Haoran;Wang, Junya;Li, Yuanyuan;Liu, Dingding;Ye, Yuanyuan;Huang, Rong;Chen, Liang;Chen, Jiedan;Yao, Mingzhe;Ma, Chunlei;Li, Sujuan
关键词:Amino acid metabolism; Camellia sinensis; chlorophyll; genetic mapping; magnesium chelatase; protein degradation