Thymol Induces Cell Death of Fusarium oxysporum f. sp. niveum via Triggering Superoxide Radical Accumulation and Oxidative Injury In Vitro
文献类型: 外文期刊
第一作者: Hao, Yini
作者: Hao, Yini;Sun, Changwei;Chen, Xuenai;Wang, Yuxiao;Yang, Lifei;Zhang, Jiao;Lu, Haiyan;Chen, Jian;Shi, Zhiqi;Zhang, Li;Huang, Sijie
作者机构:
关键词: cell death; fungicide; Fusarium oxysporum; lipid peroxidation; thymol
期刊名称:AGRONOMY-BASEL ( 影响因子:3.7; 五年影响因子:4.0 )
ISSN:
年卷期: 2023 年 13 卷 1 期
页码:
收录情况: SCI
摘要: Fusarium oxysporum f. sp. niveum (FON) causes watermelon wilt that is one of the major disease-causing yield losses of watermelon. Sustainable development of agriculture requires controlling watermelon wilt disease with good environmental performance. One important approach is to identify environmental-friendly compounds with inhibitory activity against FON. Thymol is a plant-derived compound that is safe for ecology. Little is known about the application of thymol in agriculture. In this study, we studied the inhibitory activity of thymol against FON by using morphological, physiological, and histochemical approaches. Thymol significantly inhibited colony diameter of FON in a dose-dependent manner, with EC50 at 21 mu g/mL. Thymol at 10, 21, and 35 mu g/mL decreased the fresh weight of FON mycelia by 29.0%, 50.6%, and 69.5%, respectively. Microscopic observation revealed irregular damage and loss of shape of mycelia upon thymol exposure. Thymol induced the accumulation of superoxide radical in mycelial cells and accompanied increased activity of antioxidative enzymes (SOD, superoxide dismutase; CAT, catalase). Thymol induced membrane permeability was indicated by lipid peroxidation and electrolyte leakage (increased by 29-58%) in mycelial cells. These results suggested that thymol induced oxidative damage in mycelia, which may be one of the possible reasons for thymol-induced mycelial cell death observed with fluorescent detection. Thymol decreased the production of conidia and inhibited the germination of conidia. Thymol induced superoxide radical accumulation, lipid peroxidation, and cell death in conidia as well. All of these results revealed the inhibitory activity of thymol against FON, which may have resulted from the superoxide radical-induced oxidative injury in both conidia and mycelia of FON.
分类号:
- 相关文献
作者其他论文 更多>>
-
Whole-genome resequencing reveals genetic diversity and selection characteristics of dairy goat
作者:Xiong, Jinke;Bao, Jingjing;Hu, Wenping;Shang, Mingyu;Zhang, Li
关键词:dairy goat; genetic diversity; population structure; selective signal; milk production traits
-
Oxidation of sesbania gum using an octenyl succinate-esterified composite
作者:Wang, Mingyan;Chen, Jian;Yao, Guanglong;Wang, Mingyan;Huang, Dongfen;Sun, Yue;Huan, Hengfu;Yao, Guanglong;Huan, Hengfu
关键词:Sesbania gum; modifification; property
-
Pre-harvest Nitrogen Limitation and Continuous Lighting Improve the Quality and Flavor of Lettuce (Lactuca sativa L.) under Hydroponic Conditions in Greenhouse
作者:Yang, Xiao;Hu, Jiangtao;Wang, Zheng;Huang, Tao;Zhang, Li;Peng, Jie;Yang, Qichang;Xiang, Yuting;Tomas-Barberan, Francisco A.
关键词:flavonoids; phenolic compounds; sesquiterpene lactones; LED lighting; greenhouse; lettuce taste
-
Genomic Analysis Reveals Candidate Genes Underlying Sex-Linked Eyelid Coloboma, Feather Color Traits, and Climatic Adaptation in Huoyan Geese
作者:Wen, Junhui;Zhang, Li;Gu, Hongchang;Wen, Junhui;Zhao, Xiurong;Zhang, Xinye;Ren, Xufang;Wang, Gang;Chen, Anqi;Qu, Lujiang;Yu, Jincheng;Li, Haiying;Wang, Huie;Wang, Huie
关键词:goose; eyelid coloboma; feather color; climate adaptation; genome
-
Transcription factor CsMYB77 negatively regulates fruit ripening and fruit size in citrus
作者:Zhang, Li;Xu, Yang;Li, Yanting;Zheng, Saisai;Zhao, Zhenmei;Chen, Meiling;Yi, Hualin;Wu, Juxun;Yang, Haijian
关键词:
-
Genomic insight into domestication of rubber tree
作者:Chao, Jinquan;Wu, Shaohua;Shi, Minjing;Yang, Shuguang;Zhang, Shixin;Deng, Xiaomin;Gao, Xinsheng;Zhang, Xiaofei;Hu, Yanshi;Zeng, Xia;Li, Weiguo;Tian, Wei-Min;Chao, Jinquan;Wu, Shaohua;Shi, Minjing;Yang, Shuguang;Zhang, Shixin;Deng, Xiaomin;Huang, Xiao;Gao, Xinsheng;Zhang, Xiaofei;Hu, Yanshi;Zeng, Xia;Li, Weiguo;Wu, Jilin;Hao, Bingzhong;Tian, Wei-Min;Chao, Jinquan;Wu, Shaohua;Shi, Minjing;Yang, Shuguang;Zhang, Shixin;Li, Yan;Deng, Xiaomin;Huang, Xiao;Gao, Xinsheng;Zhang, Xiaofei;Hu, Yanshi;Zeng, Xia;Li, Weiguo;Wu, Jilin;Hao, Bingzhong;Tian, Wei-Min;Xu, Xia;Du, Huilong;Fan, Xiuli;Hai, Chunyan;Kou, Liquan;Yu, Hong;Li, Jiayang;Liang, Chengzhi;Gao, Qiang;Zhang, Liangsheng;Gao, Qiang;Du, Huilong;Du, Huilong;Fan, Xiuli;Hai, Chunyan;Li, Jiayang;Liang, Chengzhi;Gao, Bin;Guo, Dong;Peng, Shiqing;Zhang, Jiao;Wang, Zhiwei;Li, Yan;Xue, Wenbo;Xu, Jiang;Wang, Xuchu;Li, Jiayang
关键词:
-
Chryseobacterium herbae Isolated from the Rhizospheric Soil of Pyrola calliantha H. Andres in Segrila Mountain on the Tibetan Plateau
作者:Zhang, Li;Li, Yan;Zhang, Li;Wang, Yan;Kong, Delong;Ma, Qingyun;Ruan, Zhiyong;Wang, Yan;Xing, Zhen;Ma, Qingyun
关键词:Chryseobacterium herbae sp. nov.; alpine ecosystems; rhizosphere soil; Gram-staining-negative; Pyrola calliantha H. Andres; genomic; shotgun proteomics