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Genetic analysis of stripe rust resistance in the common wheat line Kfa/2*Kachu under a Chinese rust environment

文献类型： 外文期刊

作者： Yin, Yuruo 1 ; Yuan, Chan 1 ; Zhang, Yichen 1 ; Li, Shunda 1 ; Bai, Bin 2 ; Wu, Ling 3 ; Ren, Yong 4 ; Singh, Ravi P. 5 ; Lan, Caixia 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Hongshan Lab, 1 Shizishan St, Wuhan 430070, Hubei, Peoples R China

2.Gansu Acad Agr Sci, Wheat Res Inst, Lanzhou 730070, Peoples R China

3.Sichuan Acad Agr Sci, Environm Friendly Crop Germplasm Innovat & Genet I, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China

4.Mianyang Inst Agr Sci, Crop Characterist Resources Creat & Utilizat Key L, Mianyang 621023, Sichuan, Peoples R China

5.Int Maize & Wheat Improvement Ctr CIMMYT, Km 45, Texcoco 56237, E Do De Mexico, Mexico

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 9 期

页码：

收录情况： SCI

摘要： Key messageWe mapped a new race-specific seedling stripe rust resistance gene on wheat chromosome 5BL and a new APR locus QYr.hazu-2BS from CIMMYT wheat line Kfa/2*Kachu.Breeding resistant wheat (Triticum aestivum) varieties is the most economical and efficient way to manage wheat stripe rust, but requires the prior identification of new resistance genes and development of associated molecular markers for marker-assisted selection. To map stripe rust resistance loci in wheat, we used a recombinant inbred line population generated by crossing the stripe rust-resistant parent 'Kfa/2*Kachu' and the susceptible parent 'Apav#1'. We employed genotyping-by-sequencing and bulked segregant RNA sequencing to map a new race-specific seedling stripe rust resistance gene, which we designated YrK, to wheat chromosome arm 5BL. TraesCS5B02G330700 encodes a receptor-like kinase and is a high-confidence candidate gene for YrK based on virus-induced gene silencing results and the significant induction of its expression 24 h after inoculation with wheat stripe rust. To assist breeding, we developed functional molecular markers based on the polymorphic single nucleotide polymorphisms in the coding sequence region of YrK. We also mapped four adult plant resistance (APR) loci to wheat chromosome arms 1BL, 2AS, 2BS and 4AL. Among these APR loci, we determined that QYr.hazu-1BL and QYr.hazu-2AS are allelic to the known pleiotropic resistance gene Lr46/Yr29/Pm39 and the race-specific gene Yr17, respectively. However, QYr.hazu-2BS is likely a new APR locus, for which we converted closely linked SNP polymorphisms into breeder-friendly Kompetitive allele-specific PCR (KASP) markers. In the present study, we provided new stripe rust resistance locus/gene and molecular markers for wheat breeder to develop rust-resistant wheat variety.

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