Effects of growth stage on the fermentation quality, microbial community, and metabolomic properties of Italian ryegrass (Lolium multiflorum Lam.) silage
文献类型: 外文期刊
作者: Fu, Zhihui 1 ; Sun, Lin 3 ; Wang, Zhijun 1 ; Liu, Jingyi 1 ; Hou, Meiling 4 ; Lu, Qiang 5 ; Hao, Junfeng 1 ; Jia, Yushan 1 ; Ge, Gentu 1 ;
作者机构: 1.Inner Mongolia Agr Univ, Coll Grassland Resources & Environm, Minist Educ, Minist Agr,Key Lab Forage Cultivat Proc & High Eff, Hohhot, Peoples R China
2.Inner Mongolia Agr Univ, Key Lab Grassland Resources, Minist Educ, Hohhot, Peoples R China
3.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China
4.Baicheng Normal Univ, Coll Life Sci, Baicheng, Peoples R China
5.Ningxia Univ, Coll Agr, Yinchuan, Peoples R China
关键词: Italian ryegrass silage; bacterial community; metabolomics profiles; SMRT; UHPLC-MS; MS
期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:5.2; 五年影响因子:6.2 )
ISSN:
年卷期: 2023 年 13 卷
页码:
收录情况: SCI
摘要: IntroductionThis study aimed to investigate the effects of different growth stages (booting period-SYK; initial flowering-SCK; full flowering-SSK) on the fermentation quality, microbial community, metabolic pathways and metabolomic characteristics of Italian ryegrass silage. MethodsSingle molecule real-time (SMRT) sequencing and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) were used to analyze bacterial communities and metabolites, respectively. ResultsAfter 60 d of fermentation, SYK had the lowest pH and the highest lactic acid content, which were significantly different from the other groups. The bacteria with the highest abundance in SYK, SCK and SSK groups were Lactiplantibacillus plantarum (63.98%), Weissella minor (28.82%) and Levilactobacillus brevis (64.81%), respectively. In addition, among the main differential metabolites in different growth stages, the number of amino acids was the most, and the corresponding metabolic pathways were mainly amino acid metabolic pathways. The biosynthesis of phenylalanine, tyrosine and tryptophan was significantly enriched (p<0.01) at booting stage and full flowering stage. Purine metabolism and ABC transporter pathway were significantly enriched at the initial flowering (p<0.001). Lactiplantibacillus plantarum had a negative correlation with xanthine and ganoderic acid F. Weissella minor had a positive correlation with D-Mannose and ganoderic acid F. Levilactobacillus brevis had a positive correlation with xanthine, and Latilactobacillus sakei had a positive correlation with cinnamic acid, D-Mannose, 2-Hydroxycinnamic acid and uridine. DiscussionIn conclusion, this study reveals the interaction mechanisms between ryegrass raw materials at different growth stages and epiphytic microorganisms during ensiling fermentation, providing new ideas for screening functional lactic acid bacteria, and laying a theoretical foundation for the production of safe and high-quality silage.
- 相关文献
作者其他论文 更多>>
-
Physicochemical characteristics and microbial community succession during oat silage prepared without or with Lactiplantibacillus plantarum or Lentilactobacillus buchneri
作者:Xiao, Yanzi;Xiao, Yanzi;Xin, Xiaoping;Xu, Lijun;Sun, Lin;Du, Shuai
关键词:lactic acid bacteria; microbial community; fermentation quality; oat silage
-
Effect of isolated lactic acid bacteria on the quality and bacterial diversity of native grass silage
作者:Bao, Jian;Ge, Gentu;Wang, Zhijun;Zhao, Muqier;Wang, Yu;Zhang, Jiawei;Jia, Yushan;Du, Shuai;Xiao, Yanzi;Sun, Lin
关键词:native grass; steppe types; lactic acid bacteria; silage quality; bacterial community
-
Effects of Cellulase and Xylanase Addition on Fermentation Quality, Aerobic Stability, and Bacteria Composition of Low Water-Soluble Carbohydrates Oat Silage
作者:Liu, Wei;Si, Qiang;Wang, Zhijun;Liu, Mingjian;Du, Shuai;Ge, Gentu;Jia, Yushan;Liu, Wei;Si, Qiang;Wang, Zhijun;Liu, Mingjian;Du, Shuai;Ge, Gentu;Jia, Yushan;Liu, Wei;Si, Qiang;Wang, Zhijun;Liu, Mingjian;Du, Shuai;Ge, Gentu;Jia, Yushan;Sun, Lin
关键词:oat silage; fermentation; aerobic stability; bacteria community; water-soluble carbohydrates
-
Varieties and ensiling: Impact on chemical composition, fermentation quality and bacterial community of alfalfa
作者:Lin, Jianyu;Li, Guanhua;Wang, Shuang;Meng, Xin;Sun, Licong;Yuan, Lin;Sun, Lin;Xu, Linbo
关键词:alfalfa; silage; chemical composition; fermentation quality; bacterial community
-
Effect of different regions on fermentation profiles, microbial communities, and their metabolomic pathways and properties in Italian ryegrass silage
作者:Fu, Zhihui;Wang, ZhiJun;Liu, Yichao;Hao, Junfeng;Gao, Cuiping;Ge, Gentu;Fu, Zhihui;Wang, ZhiJun;Liu, Yichao;Hao, Junfeng;Gao, Cuiping;Ge, Gentu;Sun, Lin
关键词:Italian ryegrass; bacterial community; metabolomic profiles; fermentation; different regions
-
Effects of Cellulase and Lactic Acid Bacteria on Ensiling Performance and Bacterial Community of Caragana korshinskii Silage
作者:Bai, Baochao;Qiu, Rui;Wang, Zhijun;Liu, Yichao;Bao, Jian;Ge, Gentu;Jia, Yushan;Bai, Baochao;Qiu, Rui;Wang, Zhijun;Liu, Yichao;Bao, Jian;Ge, Gentu;Jia, Yushan;Sun, Lin;Liu, Tingyu
关键词:lactic acid bacteria; cellulase; microbial community; fermentation characteristics; Caragana korshinskii
-
Evaluation of nano-selenium biofortification characteristics of alfalfa (Medicago sativa L.)
作者:Sun, Pengbo;Wang, Zhijun;Yuan, Ning;Lu, Qiang;Li, Yuyu;Zhang, Jiawei;Ge, Gentu;Jia, Yushan;Sun, Pengbo;Wang, Zhijun;Yuan, Ning;Lu, Qiang;Li, Yuyu;Zhang, Jiawei;Zhang, Yuhan;Ge, Gentu;Jia, Yushan;Sun, Lin
关键词:nano-selenium; alfalfa; yield; selenium accumulation; physiological characteristics