A Novel High-Affinity Potassium Transporter IbHKT-like Gene Enhances Low-Potassium Tolerance in Transgenic Roots of Sweet Potato (Ipomoea batatas (L.) Lam.)
文献类型： 外文期刊
作者： Jiang, Wei 1 ; Jin, Rong 1 ; Wang, Danfeng 1 ; Yang, Yufeng 2 ; Zhao, Peng 1 ; Liu, Ming 1 ; Zhang, Aijun 1 ; Tang, Zhonghou 1 ;
作者机构： 1.Xuzhou Inst Agr Sci, Xuzhou Sweetpotato Res Ctr, Xuzhou 221121, Jiangsu, Peoples R China
2.Henan Acad Agr Sci, Cereal Res Inst, Zhengzhou 450002, Peoples R China
关键词： sweet potato; IbHKT-like gene; potassium deficiency; transgenic roots
期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）
ISSN：
年卷期： 2022 年 11 卷 11 期
页码：
收录情况： SCI
摘要： The high-affinity potassium transporters (HKT) mediate K+-Na+ homeostasis in plants. However, the function of enhancing low-potassium tolerance in sweet potato [Ipomoea batatas (L.) Lam.] remains unrevealed. In this study, a novel HKT transporter homolog IbHKT-like gene was cloned from sweet potato, which was significantly induced by potassium deficiency stress. IbHKT-like overexpressing transgenic roots were obtained from a sweet potato cultivar Xuzishu8 using an Agrobacterium rhizogenes-mediated root transgenic system in vivo. Compared with the CK, whose root cells did not overexpress the IbHKT-like gene, overexpression of the IbHKT-like gene protected cell ultrastructure from damage, and transgenic root meristem cells had intact mitochondria, endoplasmic reticulum, and Golgi dictyosomes. The steady-state K+ influx increased by 2.2 times in transgenic root meristem cells. Overexpression of the IbHKT-like gene also improved potassium content in the whole plant, which increased by 63.8% compared with the CK plants. These results could imply that the IbHKT-like gene, as a high-affinity potassium transporter gene, may play an important role in potassium deficiency stress responses.
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