A Novel WRKY Transcription Factor from Ipomoea trifida, ItfWRKY70, Confers Drought Tolerance in Sweet Potato
文献类型： 外文期刊
作者： Sun, Sifan 1 ; Li, Xu 1 ; Gao, Shaopei 1 ; Nie, Nan 1 ; Zhang, Huan 1 ; Yang, Yufeng 1 ; He, Shaozhen 1 ; Liu, Qingchang 1 ; Zhai, Hong 1 ;
作者机构： 1.China Agr Univ, Key Lab Sweet Potato Biol & Biotechnol, Minist Agr & Rural Affairs,Lab Crop Heterosis & U, Beijing Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China
2.Zhengzhou Univ, Cereal Crops Res Inst, Henan Acad Agr Sci, Postgrad T&R Base, Zhengzhou 450000, Peoples R China
3.Zhengzhou Univ, Sch Agr Sci, Zhengzhou 450000, Peoples R China
关键词： sweet potato; Ipomoea trifida; ItfWRKY70; drought tolerance; ABA; stomatal aperture
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 2 期
页码：
收录情况： SCI
摘要： WRKY transcription factors are one of the important families in plants, and have important roles in plant growth, abiotic stress responses, and defense regulation. In this study, we isolated a WRKY gene, ItfWRKY70, from the wild relative of sweet potato Ipomoea trifida (H.B.K.) G. Don. This gene was highly expressed in leaf tissue and strongly induced by 20% PEG6000 and 100 mu M abscisic acid (ABA). Subcellar localization analyses indicated that ItfWRKY70 was localized in the nucleus. Overexpression of ItfWRKY70 significantly increased drought tolerance in transgenic sweet potato plants. The content of ABA and proline, and the activity of SOD and POD were significantly increased, whereas the content of malondialdehyde (MDA) and H2O2 were decreased in transgenic plants under drought stress. Overexpression of ItfWRKY70 up-regulated the genes involved in ABA biosynthesis, stress-response, ROS-scavenging system, and stomatal aperture in transgenic plants under drought stress. Taken together, these results demonstrated that ItfWRKY70 plays a positive role in drought tolerance by accumulating the content of ABA, regulating stomatal aperture and activating the ROS scavenging system in sweet potato.
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