文献类型: 会议论文
第一作者: K. Iitani
作者: K. Iitani 1 ; M. Naisierding 1 ; T. Sato 1 ; K. Toma 2 ; T. Arakawa 2 ; K. Mitsubayashi 1 ;
作者机构: 1.Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
2.Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
会议名称: PRiME Joint International Meeting of the Electrochemical Society, the Electrochemical Society of Japan, and the Korean Electrochemical Society
主办单位:
页码: 53-57
摘要: We developed Sniff-cam (bio-fluorometric gas-imaging system) for visualization of breath acetaldehyde (ACE) which is produced by ethanol metabolism in liver after drinking. Sniff-cam employed an alcohol dehydrogenase (ADH) immobilized cotton mesh with reduced nicotinamide adenine dinucleotide (NADH) for imaging of gaseous ACE distribution. NADH is consumed by ADH-mediated reduction of ACE, and its concentration and that of ACE are correlated. Therefore, imaging of gaseous ACE distribution was achieved by exciting NADH with an ultraviolet light-emitting diode array (340 nm wavelength) and recording the emitted fluorescence from NADH (490 nm wavelength) by a high sensitive camera. Decrement of fluorescence at around the gas outlet was observed when ACE vapor was introduced. This result demonstrates that the Sniff-cam could be applied for imaging of breath ACE.
分类号: TP212-53
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