您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of Low Oxygen Tension during Expansion on Chondrogenic Potential of Osteoarthritic Chondrocytes

文献类型： 会议论文

第一作者： Kevin A. Davis

作者： Kevin A. Davis 1 ; James H. Henderson 1 ; Jing Wang 1 ;

作者机构： 1.Department of Biomedical and Chemical Engineering Syracuse Biomaterials Institute Syracuse University Syracuse, New York, USA

关键词： OA chondrocytes;low oxygen tension;dedifferentiation

会议名称： Annual Northeast Bioengineering Conference

主办单位：

页码： 84-85

摘要： Primary human chondrocytes, although a promising cell source for cartilage tissue engineering, tend to dedifferentiate and lose their characteristic gene expression and protein production during monolayer, expansion. Low O_2 tension in vitro culture has been studied to examine its effect on preventing dedifferentiation, and results have been conflicting. In particular, low O_2 expansion effects on human osteoarthritis (OA) chondrocytes are poorly understood. In this study, we expanded chondrocytes collected from patients with osteoarthritis (OA) under normal (21%) and low (5%) O_2 tension then continued with micromass culture under 21% O_2 for 3 weeks. Our results suggest that low O_2 condition may promote chondrogenic characteristic gene expression in monolayer cells. But improved ECM production, which was seen earlier in animal models, may not be relevant to human OA chondrocytes. Further examination of the mechanical properties of the engineered pellets is needed to confirm the effects of low O_2 expansion on human OA chondrocytes and their use in cartilage tissue engineering.

分类号： q81-53

  • 相关文献

[1]Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4. Li, Xiangchen,Guo, Yu,Yao, Yaxin,Ma, Yuehui,Liu, Changqing,Guan, Weijun,Guo, Yu,Liu, Changqing,Hua, Jinlian. 2016

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台