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Fatty acid analysis of the transgenic tobacco expressing a delta 6-desaturase gene from Microula sikkimensis

文献类型: 会议论文

第一作者: Shujuan Wu

作者: Shujuan Wu 1 ; Lijing Zhang 1 ; Xiaolong Chen 1 ; Xiumei Miao 1 ; Decao Niu 1 ; Hua Fu 1 ;

作者机构: 1.The State Key Laboratory of Grassland Farming Systems, College of Pastoral Agriculture Science and Technology, Lanzhou University, P O Box 61, Lanzhou 730000, People's Republic of China

关键词: Delta 6-dcsaturasc;transgenic tobacco;fatty acid analysis;y-linolenic acid;octadecatetraenoic acid

会议名称: International Grassland Congress

主办单位:

页码: 389-390

摘要: Linolenic acid (GLA, 18:3~(A6,9,12)) and octadccatetraenoic d(OTA, 18:4~(A6,9,12)) are important polyunsaturated toy acids (PUF As), which have been proved to be benefit forhuman health (Fan and Chapkin 1998; Whelan 2009). Currently, fish arc the predominant source of PUFAs. However, with the growth of world's population and the more nutrition requirements, fishery resources are shrinking. Alternative sources of PUFAs are being investigated (Truksa et al. 2009). The major oil crops do not contain GLAand OTA, only several plant species contain these important fatty acids in their leaf lipids and seed oils (Zhou at al. 2006). Genetic modification of oil crops may be an effective approach to produce these fatty acids. This process requires an enzyme -delta 6-desaturase, which can introduce a double bond at the delta 6 position (Meesa-pyodsuk and Qiu 2012). Microula sikkimensis is a kind of rare wild oil plant, which is widely distributed in Bhutan, Sikkim, Nepal and the northwest region of China (Cao and Suo 2010). Fu et al. (1997) reported that unsaturated fatty acids made up 86.5% of total fatty acids, and that GLA accounted for 6.4% of fatty acids in M. sikkimensis seeds that is known as a new source of GLA.

分类号: S812-532

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