文献类型： 会议论文

第一作者： Niels-Christian Reichardt

作者： Niels-Christian Reichardt ¹ ;

作者机构： 1.Biofunctional Nanomateriak Unit, CICbiomaGUNE, Paseo Miramon 182, 22000 San Sebastian/Spain

会议名称： International Carbohydrate Symposium

主办单位：

页码： 39-39

摘要： Glycan arrays have emerged as versatile platforms in Functional Glycomics for the high-throughput screening of binding specificities for lectins and antibodies or to determine the substrate requirements of carbohydrate processing enzymes. A remaining major bottleneck for a more extended use of glycan arrays is the supply with sufficiently pure and well-characterized ligands. Focusing on N-glycans our laboratory has combined the synthesis of N-glycan core structures with their enzymatic modification on the chip using recombinant glycosyltransferases to generate a library of structures with systematic variations in number of antennae, terminal sugars and core modifications. Surface-based MALDI-Tof MS methods have been developed to evaluate the enzymatic on-chip glycosylation and as a powerful tool to analyze biomass degrading enzymes in environmental samples.

分类号： o629