Evaluation of Transgenic Sweet Orange (Citrus sinenis Osbeck)Containing Bivalent Anti-bacterial Peptide Genes ShivaA and CecropinB
文献类型： 会议论文
第一作者： Yongrui He
作者： Yongrui He 1 ; Aihong Peng 1 ; Lanzhen Xu 1 ; Tiangang Lei 2 ; Xiaofeng Liu; Lixiao Yao; Shanchun Chen; Shirong Jia; Yixiong Tang;
作者机构： 1.Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, P. R. China National Citrus Engineering Research Center, Chongqing 400712, P. R. China National Center for Citrus Variety Improvement, Chongqing 400712, P. R. China
2.Citrus Research Institute, Chinese Academy o
关键词： Mature axillary bud;Transformation;Horticultural characteristics;Canker resistance
会议名称： International citrus congress
主办单位：
页码： 272-280
摘要： The axillary buds from mature branch of Jincheng Orange (Citrus sinenis Osbeck) and Newhall navel orange (C. sinenis Osbeck) were used for Agrobacterium-based transformation. PCR and Southern blotting analysis verified that the ShivaA and CecropinB genes had been integrated into the genome of transgenic plants. The transgenic plants began to blossom and to bear fruits in the 3rd year after grafted on trifoliate orange (Poncirus trifoliata Raf) rootstocks in greenhouse. Through evaluating in greenhouse, no significant differences were observed on the total soluble solids, total acidity, reducing sugar and fruit shape index of characteristics between transgenic and non-transgenic mature lines. Extract of transgenic citrus leaves could suppress the growth of citrus canker pathogen (Xanthomonas axonopodis pv. citri) in vitro, which suggests that the ShivaA and CecropinB had expressed efficiently in some transgenic citrus and exhibited anti-bacterial activity. By artificially inoculating with the canker pathogen in greenhouse and field, the different resistance capabilities to canker were observed among transgenic and control lines, and we had selected 11 significantly enhanced canker resistance lines from 40 transgenic lines.
分类号： S666`S666
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