HSC70/HSP40 OVEREXPRESSION IN VIRAL VECTOR-PRODUCING SF9 CELLS IMPACTS AAV PRODUCTIVITY AND PRODUCT QUALITY
文献类型: 会议论文
第一作者: Juan Aponte-Ubillus
作者: Juan Aponte-Ubillus 1 ; Jim Liu 1 ; Daniel Barajas 1 ; Joseph Peltier 2 ; Javier Femenia 1 ;
作者机构: 1.Drug Substance Technologies, BioMarin Pharmaceutical Inc
2.Innovation and External Partnership, BioMarin Pharmaceutical Inc
关键词: Viral vector;Heat shock protein;Sf9-baculovirus
会议名称: [ "Cell culture engineering conference" , "Cell Culture Engineering Conference"]
主办单位:
页码: 257-257
摘要: Cytosolic heat shock proteins such as HSC70 and HSP40 play an important role in protein folding and cell stress relief, making them potential targets for cell line engineering in production cell factories. We hypothesized that increasing amounts of these key chaperones in Sf9 cells during AAV vector production might lead to improved recombinant vector productivity and/or product quality, conceivably by increasing the availability of active structural (VP1-3) and non-structural (small and large Rep) proteins and by ameliorating possible impacts of proteotoxic stress.
分类号: q813-53
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