您好,欢迎访问中国农业科学院 机构知识库!

HSC70/HSP40 OVEREXPRESSION IN VIRAL VECTOR-PRODUCING SF9 CELLS IMPACTS AAV PRODUCTIVITY AND PRODUCT QUALITY

文献类型: 会议论文

第一作者: Juan Aponte-Ubillus

作者: Juan Aponte-Ubillus 1 ; Jim Liu 1 ; Daniel Barajas 1 ; Joseph Peltier 2 ; Javier Femenia 1 ;

作者机构: 1.Drug Substance Technologies, BioMarin Pharmaceutical Inc

2.Innovation and External Partnership, BioMarin Pharmaceutical Inc

关键词: Viral vector;Heat shock protein;Sf9-baculovirus

会议名称: [ "Cell culture engineering conference" , "Cell Culture Engineering Conference"]

主办单位:

页码: 257-257

摘要: Cytosolic heat shock proteins such as HSC70 and HSP40 play an important role in protein folding and cell stress relief, making them potential targets for cell line engineering in production cell factories. We hypothesized that increasing amounts of these key chaperones in Sf9 cells during AAV vector production might lead to improved recombinant vector productivity and/or product quality, conceivably by increasing the availability of active structural (VP1-3) and non-structural (small and large Rep) proteins and by ameliorating possible impacts of proteotoxic stress.

分类号: q813-53

  • 相关文献

[1]Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels. Yu, Gui Mei,Zu, Shu Long,Zhou, Wei Wei,Wang, Xi Jun,Shuai, Lei,Wang, Xue Lian,Ge, Jin Ying,Bu, Zhi Gao. 2017

[2]Characterization and binding analysis of a microneme adhesive repeat domain-containing protein from Toxoplasma gondii. Gong, Haiyan,Kobayashi, Kyousuke,Sugi, Tatsuki,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Horimoto, Taisuke,Akashi, Hiroomi,Kato, Kentaro,Gong, Haiyan,Sugi, Tatsuki,Takemae, Hitoshi,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Xuan, Xuenan,Kato, Kentaro. 2014

[3]Alleviative effect of selenium on inflammatory damage caused by lead via inhibiting inflammatory factors and heat shock proteins in chicken testes. Wang, Yan,Wang, Kexin,Huang, He,Teng, Xiaohua,Gu, Xianhong.

[4]Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.). Li, Zhenyi,Long, Ruicai,Zhang, Tiejun,Wang, Zhen,Zhang, Fan,Yang, Qingchuan,Kang, Junmei,Sun, Yan.

[5]Over-expression of LlHsfA2b, a lily heat shock transcription factor lacking trans-activation activity in yeast, can enhance tolerance to heat and oxidative stress in transgenic Arabidopsis seedlings. Xin, Haibo,Zhong, Xionghui,Yi, Mingfang,Xin, Haibo,Zhang, Hua,Dong, Aixiang,Cong, Richen,Lian, Qinglong,Cao, Li.

[6]Effects of maternal dietary manganese and incubation temperature on hatchability, antioxidant status, and expression of heat shock proteins in chick embryos. Zhu, Y. W.,Lu, L.,Li, W. X.,Zhang, L. Y.,Luo, X. G.,Zhu, Y. W.,Ji, C.,Lin, X.,Liu, H. C.,Odle, J..

[7]The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells. Hu, Han,Zhang, Yangdong,Zheng, Nan,Cheng, Jianbo,Wang, Jiaqi,Hu, Han,Zhang, Yangdong,Zheng, Nan,Cheng, Jianbo,Wang, Jiaqi,Hu, Han,Zhang, Yangdong,Zheng, Nan,Cheng, Jianbo,Wang, Jiaqi.

[8]Molecular cloning and characterization of the MsHSP17.7 gene from Medicago sativa L.. Li, Zhen-yi,Long, Rui-cai,Zhang, Tie-jun,Yang, Qing-chuan,Kang, Jun-mei.

[9]Three Heat Shock Protein Genes from Bactrocera (Tetradacus) minax Enderlein: Gene Cloning, Characterization, and Association with Diapause. Lu, Z. C.,Wang, L. H.,Zhang, G. F.,Wan, F. H.,Guo, J. Y.,Wan, F. H.,Guo, J. Y.,Wang, L. H.,Yu, H.,Wang, J. B.. 2014

[10]cDNA-AFLP analysis reveals heat shock proteins play important roles in mediating cold, heat, and drought tolerance in Ammopiptanthus mongolicus. Guo, Huiming,Li, Zhaochun,Zhou, Meiliang,Cheng, Hongmei.

作者其他论文 更多>>