Effect of N-terminal and C-terminal Deletion of Cry1Ie of Bacillus thuringiensis on its Expression and Purification
文献类型: 会议论文
第一作者: Huicong Shen
作者: Huicong Shen 1 ; Chunlu Zhang 1 ; Shuyuan Guo 1 ; Jie Zhang 2 ; Fuping Song 2 ;
作者机构: 1.School of Life Science & Technology, Beijing Institute of Technology
2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences
关键词: Bacillus thuringienesis;Insecticidal protein;Cry1Ie;Mutant
会议名称: iCBBE 2009
主办单位:
页码: 776-779
摘要: Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.
分类号: G202-53
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