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A comprehensive study of the differences in protein expression and chemical constituents in tea leaves (Camellia sinensis var. sinensis) with different maturity using a combined proteomics and metabolomics method

文献类型: 外文期刊

作者: Sun, Zhen 1 ; Chen, Dan 2 ; Zhu, Liyao 1 ; Zhao, Yanni 3 ; Lin, Zhi 2 ; Li, Xianzhen 1 ; Dai, Weidong 2 ;

作者机构: 1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Liaoning, Peoples R China

2.Chinese Acad Agr Sci, Tea Res Inst, Key Lab Biol Genet & Breeding Special Econ Anim &, Minist Agr & Rural Affairs, Hangzhou 310008, Zhejiang, Peoples R China

3.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China

关键词: Tea; Proteomics; Metabolomics; Maturity; Metabolic pathways

期刊名称:FOOD RESEARCH INTERNATIONAL ( 影响因子:7.425; 五年影响因子:7.716 )

ISSN: 0963-9969

年卷期: 2022 年 157 卷

页码:

收录情况: SCI

摘要: The maturity of tea leaves has a great influence on the flavor quality and commercial price of tea. In this work, a combined proteomics and metabolomics analysis was applied to investigate the differences in protein expression and metabolites among tea leaves with different maturity. Integrated analysis showed that there were significant differences in 112 nonvolatile components related to the pathways of photosynthesis, glycolysis, tricarboxylic acid cycle, and the biosynthesis of amino acids, phenylpropanoids and flavonoids. The bud had higher expression levels of most enzymes related to the biosynthesis of amino acids, phenylpropanoids, and flavonoids, leading to higher levels of amino acids, most flavanols, and procyanidins compared with the leaves. The 1st leaf showed a higher expression level of flavonol synthase, which produces higher levels of flavonol-3-glycosides. This study offers deep insight into the maturity of tea at both the protein and metabolite levels and provides a guideline for tea manufacturing.

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