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A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew

文献类型： 外文期刊

作者： He, Huagang 1 ; Chen, Zhaozhao 1 ; Fan, Renchun 2 ; Zhang, Jie 3 ; Zhu, Shanying 4 ; Wang, Jiale 1 ; Zhang, Qianyuan 1 ; Gao, Anli 5 ; Gong, Shuangjun 6 ; Zhang, Lu 7 ; Li, Yanan 2 ; Zhao, Yitong 2 ; Krattinger, Simon G. 8 ; Shen, Qian-Hua 2 ; Li, Hongjie 9 ; Wang, Yajun 7 ;

作者机构： 1.Jiangsu Univ, Sch Life Sci, Zhenjiang, Peoples R China

2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China

3.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol Res, Chengdu, Peoples R China

4.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang, Peoples R China

5.Henan Univ, Sch Life Sci, Kaifeng, Peoples R China

6.Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Wuhan, Peoples R China

7.Chinese Acad Sci, Inst Plant Physiol & Ecol, Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet,Key Lab Plant Design, Shanghai, Peoples R China

8.King Abdullah Univ Sci & Technol KAUST, Plant Sci Program, Biol & Environm Sci & Engn Div BESE, Thuwal, Saudi Arabia

9.Xianghu Lab, Inst Biotechnol, Hangzhou, Peoples R China

10.Chinese Acad Agr Sci, Inst Crop Sci, Natl Engn Lab Crop Mol Breeding, Beijing, Peoples R China

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.7； 五年影响因子：16.1 ）

ISSN：

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the introgressed segments hinders the cloning of the introgressed genes. Here, we have cloned the powdery mildew resistance gene Pm13, which is introgressed into wheat from Aegilops longissima, using a method that combines physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis of ethyl methanesulfonate (EMS)-induced loss-of-function mutants. Pm13 encodes a kinase fusion protein, designated MLKL-K, with an N-terminal domain of mixed lineage kinase domain-like protein (MLKL_NTD domain) and a C-terminal serine/threonine kinase domain bridged by a brace. The resistance function of Pm13 is validated through transient and stable transgenic complementation assays. Transient over-expression analyses in Nicotiana benthamiana leaves and wheat protoplasts reveal that the fragment Brace-Kinase(122-476) of MLKL-K is capable of inducing cell death, which is dependent on a functional kinase domain and the three alpha-helices in the brace region close to the N-terminus of the kinase domain.

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