文献类型： 外文期刊

作者： Li, Guo-Bang ¹ ; Liu, Jie ¹ ; He, Jia-Xue ¹ ; Li, Gao-Meng ¹ ; Zhao, Ya-Dan ¹ ; Liu, Xiao-Ling ¹ ; Hu, Xiao-Hong ¹ ; Zhang, Xin ¹ ; Wu, Jin-Long ¹ ; Shen, Shuai ¹ ; Liu, Xin-Xian ¹ ; Zhu, Yong ¹ ; He, Feng ⁴ ; Gao, Han ⁵ ; Wang, He ¹ ; Zhao, Jing-Hao ¹ ; Li, Yan ¹ ; Huang, Fu ¹ ; Huang, Yan-Yan ¹ ; Zhao, Zhi-Xue ¹ ; Zhang, Ji-Wei ¹ ; Zhou, Shi-Xin ¹ ; Ji, Yun-Peng ¹ ; Pu, Mei ¹ ; He, Min ¹ ; Chen, Xuewei ¹ ; Wang, Jing ¹ ; Li, Weitao ² ; Wu, Xian-Jun ² ; Ning, Yuese ⁴ ; Sun, Wenxian ⁵ ; Xu, Zheng-Jun ² ; Wang, Wen-Ming ¹ ; Fan, Jing ¹ ;

作者机构： 1.Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu 611130, Peoples R China

2.Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China

3.Mianyang Normal Univ, Ecol Secur & Protect Key Lab Sichuan Prov, Mianyang 621023, Peoples R China

4.Inst Plant Protect, Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

5.China Agr Univ, Coll Plant Protect, Beijing 100193, Peoples R China

6.China Agr Univ, Minist Agr Key Lab Pest Monitoring & Green Managem, Beijing 100193, Peoples R China

7.Yazhouwan Natl Lab, Sanya 572024, Peoples R China

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.9 ）

ISSN： 1040-4651

年卷期： 2024 年 36 卷 5 期

页码：

收录情况： SCI

摘要： The flower-infecting fungus Ustilaginoidea virens causes rice false smut, which is a severe emerging disease threatening rice (Oryza sativa) production worldwide. False smut not only reduces yield, but more importantly produces toxins on grains, posing a great threat to food safety. U. virens invades spikelets via the gap between the 2 bracts (lemma and palea) enclosing the floret and specifically infects the stamen and pistil. Molecular mechanisms for the U. virens-rice interaction are largely unknown. Here, we demonstrate that rice flowers predominantly employ chitin-triggered immunity against U. virens in the lemma and palea, rather than in the stamen and pistil. We identify a crucial U. virens virulence factor, named UvGH18.1, which carries glycoside hydrolase activity. Mechanistically, UvGH18.1 functions by binding to and hydrolyzing immune elicitor chitin and interacting with the chitin receptor CHITIN ELICITOR BINDING PROTEIN (OsCEBiP) and co-receptor CHITIN ELICITOR RECEPTOR KINASE1 (OsCERK1) to impair their chitin-induced dimerization, suppressing host immunity exerted at the lemma and palea for gaining access to the stamen and pistil. Conversely, pretreatment on spikelets with chitin induces a defense response in the lemma and palea, promoting resistance against U. virens. Collectively, our data uncover a mechanism for a U. virens virulence factor and the critical location of the host-pathogen interaction in flowers and provide a potential strategy to control rice false smut disease. The rice false smut fungus secretes a glycoside hydrolase protein to degrade chitin and block dimerization of host chitin receptors suppressing pattern-triggered immunity and infecting flowers.