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GhSINA1, a SEVEN in ABSENTIA ubiquitin ligase, negatively regulates fiber development in Upland cotton

文献类型： 外文期刊

作者： Yang, Xiao-Qing 1 ; Li, Wei 1 ; Ren, Zhong-Ying 1 ; Zhao, Jun-Jie 1 ; Li, Xin-Yang 1 ; Wang, Xing-Xing 1 ; Pei, Xiao-Yu 1 ; Liu, Yan-Gai 1 ; He, Kun-Lun 1 ; Zhang, Fei 1 ; Ma, Xiong-Feng 1 ; Yang, Dai-Gang 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang 455000, Peoples R China

2.Zhengzhou Univ, Sch Agr Sci, Natl Key Lab Cotton Biobreeding & Integrated Utili, Zhengzhou Res Base, Zhengzhou 450001, Peoples R China

3.Chinese Acad Agr Sci, Western Agr Res Ctr, Changji 831100, Peoples R China

关键词： Ubiquitination; Ubiquitin ligase; SINA; Cotton; Fiber

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 201 卷

页码：

收录情况： SCI

摘要： Protein ubiquitination is essential for plant growth and responses to the environment. The SEVEN IN ABSENTIA (SINA) ubiquitin ligases have been extensively studied in plants, but information on their roles in fiber development is limited. Here, we identified GhSINA1 in Upland cotton (Gossypium hirsutum), which has a conserved RING finger domain and SINA domain. Quantitative real-time PCR (qRT-PCR) analysis showed that GhSINA1 was preferentially expressed during fiber initiation and elongation, especially during initiation in the fuzzless-lintless cotton mutant. Subcellular localization experiments indicated that GhSINA1 localized to the nucleus. In vitro ubiquitination analysis revealed that GhSINA1 has E3 ubiquitin ligase activity. Ectopic overexpression of GhSINA1 in Arabidopsis thaliana reduced the number and length of root hairs and trichomes. Yeast two-hybrid (Y2H), firefly luciferase complementation imaging (LCI), and bimolecular fluorescence complementation (BiFC) assays demonstrated that the GhSINA1 proteins could interact with each other to form homodimers and heterodimers. Overall, these results suggest that GhSINA1 may act as a negative regulator in cotton fiber development through homodimerization and heterodimerization.

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