A Genome-Wide Analysis of Serine Protease Inhibitors in Cydia pomonella Provides Insights into Their Evolution and Expression Pattern
文献类型： 外文期刊
作者： Wu, Qiang 1 ; Xing, Longsheng 2 ; Du, Min 3 ; Huang, Cong 1 ; Liu, Bo 1 ; Zhou, Hongxu 3 ; Liu, Wanxue 4 ; Wan, Fanghao 1 ; Qian, Wanqiang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Minist Agr & Rural Affairs, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,G, Shenzhen 518120, Peoples R China
2.Hebei Univ, Inst Life Sci & Green Dev, Coll Life Sci, Hebei Basic Sci Ctr Biot Interact, Baoding 071000, Peoples R China
3.Qingdao Agr Univ, Sino Australian Joint Res Inst Agr & Environm Hlth, Coll Plant Hlth & Med, Shandong Prov Key Lab Integrated Control Plant Dis, Qingdao 266109, Peoples R China
4.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China
关键词： serine protease inhibitor; melanization; Cydia pomonella; innate immunity; alternative splicing
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 22 期
页码：
收录情况： SCI
摘要： Serine protease inhibitors (serpins) appear to be ubiquitous in almost all living organisms, with a conserved structure and varying functions. Serpins can modulate immune responses by negatively regulating serine protease activities strictly and precisely. The codling moth, Cydia pomonella (L.), a major invasive pest in China, can cause serious economic losses. However, knowledge of serpin genes in this insect remain largely unknown. In this study, we performed a systematic analysis of the serpin genes in C. pomonella, obtaining 26 serpins from the C. pomonella genome. Subsequently, their sequence features, evolutionary relationship, and expression pattern were characterized. Comparative analysis revealed the evolution of a number of serpin genes in Lepidoptera. Importantly, the evolutionary relationship and putative roles of serpin genes in C. pomonella were revealed. Additionally, selective pressure analysis found amino acid sites with strong evidence of positive selection. Interestingly, the serpin1 gene possessed at least six splicing isoforms with distinct reactive-center loops, and these isoforms were experimentally validated. Furthermore, we observed a subclade expansion of serpins, and these genes showed high expression in multiple tissues, suggesting their important roles in C. pomonella. Overall, this study will enrich our knowledge of the immunity of C. pomonella and help to elucidate the role of serpins in the immune response.
- 相关文献
作者其他论文 更多>>
-
Inhibition of the invasive plant Ambrosia trifida by Sigesbeckia glabrescens extracts
作者：Yin, Lijuan;Zhang, Yuxin;Wangchen, Jiaozi;Wan, Fanghao;Liu, Bo;Qian, Wanqiang;Yin, Lijuan;Zhang, Guangzhong;Zhao, Haoyu
关键词：Ambrosia trifida; Sigesbeckia glabrescens extracts; Decreased carbon and nitrogen metabolism; Oxidative stress; Major allelopathic metabolites
-
Study on Rapid Quantitative Detection of Soil MPs Based on Terahertz Time-Domain Spectroscopy
作者：Xu, Lijia;Feng, Yanqi;Feng, Ao;Yang, Yuping;Chen, Yanjun;Wu, Zhijun;Wang, Yuchao;Zhao, Yongpeng;Yang, Yuping;Liu, Bo;Yang, Ning;Ma, Wei;He, Yong
关键词：
-
Influence of Mikania micrantha Kunth Flavonoids on Composition of Soil Microbial Community
作者：Yang, Qilin;Cui, Wenyang;Guan, Zijun;Wang, Zhenzhen;Jahan, Israt;Li, Ping;Yan, Jian;Qin, Feng;Qiao, Xi;Liu, Bo
关键词：
Mikania micrantha ; flavonoids; soil microbial communities; arbuscular mycorrhizal fungi (AMF); invasive plant management -
The transformer gene controls sexual development in Drosophila suzukii
作者：Yan, Ying;Zhao, Jing;Schwirz, Jonas;Borghesi, Cristina;Schetelig, Marc F.;Liu, Conghui;Liu, Bo;Qian, Wanqiang;Wan, Fanghao
关键词：courtship behavior; gene splicing; pigmentation; sex determination; spotted-wing
Drosophila ; Tetracycline-Off system -
Pre-mRNA processing factors differentially impact coordination between co-transcriptional cleavage and transcription termination
作者：Jin, Xianhao;Li, Juzuo;Lu, Wenqin;Shu, Yi;Liu, Bo;Long, Yanping;Zhu, Xinlong;Zhai, Jixian;Deng, Xian;Cao, Xiaofeng;Deng, Xian;Cao, Xiaofeng;Wei, Yanping;Fei, Qili;Liu, Zhijian;Xia, Yiji;Xia, Yiji;Li, Qingshun Q.;Michaels, Scott D.;Lenhard, Michael
关键词：
-
Identification of Thalictrum squarrosum as an alternate host for Puccinia triticina and pathogen analysis of Thalictrum squarrosum rust
作者：Zhao, Na;Huang, Liang;Ren, Jun;Yi, Ting;Li, Hongfu;Zhang, Hao;Liu, Bo;Gao, Li;Chen, Wanquan;Liu, Taiguo;Zhao, Na;Huang, Liang;Ren, Jun;Yi, Ting;Li, Hongfu;Zhang, Hao;Gao, Li;Chen, Wanquan;Liu, Taiguo;Zhang, Mengya;Yan, Hongfei;Liu, Taiguo
关键词：wheat leaf rust;
Puccinia triticina ;Thalictrum squarrosum ; alternate host; sexual reproduction -
Integrated omic analysis provides insights into how Cuscuta australis inhibits the growth and reproduction of Xanthium spinosum
作者：Wang, Yunxia;Bu, Hubai;Gu, Xin;Wang, Xinpu;Liu, Wanxue
关键词：
Xanthium spinosum ;Cuscuta australis ; Growth and development; Reproductive performance; Metabolic pathways
