您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae)

文献类型： 外文期刊

作者： Wang, Liuyang 1 ; Guo, Pei 2 ; Zhang, Xiaofang 2 ; Duan, Yun 4 ; Ning, Jun 3 ; Zhang, Tao 2 ; Yang, Xinling 1 ;

作者机构： 1.China Agr Univ, Coll Sci, Innovat Ctr Pesticide Res, Dept Appl Chem, Beijing 100193, Peoples R China

2.Minist Agr & Rural Affairs, China IPM Innovat Ctr Hebei Prov, Int Sci & Technol Joint Res Ctr IPM Hebei Prov, Plant Protect Inst,HAAFS Key Lab IPM Crops Norther, Baoding 071000, Peoples R China

3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

4.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China

关键词： desaturases; unsaturated fatty acids; RNA interference; mating behavior; yeast expression; biosyntheticpathway

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 19 期

页码：

收录情况： SCI

摘要： In moths, sex pheromones are synthesized in pheromone glands (PGs) by a variety of enzymes. Desaturases (DESs) are critical for the introduction of double bonds into pheromones. In Mythimna loreyi, the specific DESs involved in sex pheromone biosynthesis remain unclear. In this study, we identified and characterized 25 putative DESs from the M. loreyi genome. Nineteen of them were expressed in the female PGs, with seven showing significant upregulation in response to pheromone biosynthesis activating neuropeptide (PBAN). RNAi-based knockdown of MlorDES2 significantly reduced the titer of pheromone components by 58.6-85.9%, while knockdown of MlorDES9 specifically reduced the production of (Z)-9-tetradecenyl acetate. Functional verification in yeast revealed that MlorDES2 and MlorDES9 exhibited Delta 11 and Delta 9 desaturase activities, respectively. Taken together, these results collectively demonstrate that MlorDES2 and MlorDES9 are involved in sex pheromone biosynthesis of M. loreyi, suggesting that DESs could be used as potential targets for pest management.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台