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Isolation and characterization of the virulent porcine epidemic diarrhea virus strain CHGS20 in China

文献类型： 外文期刊

作者： Wang, Jingyi 1 ; Yin, Shuanghui 2 ; Pan, Sumin 1 ; Zhang, Xiaoqing 1 ; Fang, Yupeng 1 ; Zhao, Tian 1 ; Li, Li 3 ; Zhang, Jie 1 ; Tarasiuk, Kazimierz 4 ; Liu, Yongsheng 1 ; Pejsak, Zygmunt 4 ; Yang, Shunli 1 ;

作者机构： 1.Hebei Normal Univ Sci & Technol, Coll Anim Sci & Technol, Key Lab Prevent Vet Med Hebei, HNUST, Qinhuangdao 066004, Hebei, Peoples R China

2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China

3.Shandong BaiCe Test Co LTD, Jinan 250101, Shandong, Peoples R China

4.Agr Univ Krakow, Vet Fac, Krakow, Poland

关键词： porcine epidemic diarrhea virus; isolation; phylogenetic analysis; electric cell-substrate impedance sensing

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.5； 五年影响因子：2.2 ）

ISSN： 0304-8608

年卷期： 2025 年 170 卷 8 期

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED), which threatens the swine industry worldwide. Although commercial PEDV vaccines are available, the disease continues to plague the swine industry in China. Therefore, isolating PEDV from clinical samples is beneficial for conducting molecular epidemiological investigations, developing effective vaccines and diagnostic methods, and investigating pathogenic mechanisms. In this study, a genotype II PEDV strain, CHGS20, was isolated from a small-intestine sample collected in 2020 from a PEDV-infected piglet and propagated in Vero cells. Virus production in cell culture was confirmed by cytopathology, immunofluorescence assay, and transmission electron microscopy. The infectious virus titers during the first 10 passages ranged from 103.3 to 107.8 TCID50 per mL. The inactivated CHGS20 virus was found to induce a potent neutralizing antibody response in immunized mice, with a mean neutralizing antibody titer of 1:136 against the homologous strain and 1:46 against the genotype I strain JX18. The dynamics of the cytopathic effect (CPE) induced by CHGS20 and JX18 were analyzed using an electric cell-substrate impedance sensing system. The results indicated that this CPE assay has the potential to be used to assess the neutralizing activity of antibodies and facilitate the development of antiviral agents. These findings will be helpful for future understanding, control, and prevention of PED.

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