文献类型： 外文期刊

作者： Li, Haiyan ¹ ; Xie, Jun ¹ ; Gao, Yongmeng ¹ ; Wang, Xuemei ¹ ; Qin, Li ² ; Ju, Wei ³ ; Roberts, Jeremy A. ⁴ ; Cheng, Beijiu ¹ ; Zhang, Xuebin ⁵ ; Lu, Xiaoduo ¹ ;

作者机构： 1.Anhui Agr Univ, Sch Life Sci, Natl Engn Lab Crop Stress Resistance, Hefei 230036, Peoples R China

2.Qilu Normal Univ, Inst Adv Agr Technol, Jinan 250200, Peoples R China

3.Nanbei Agr Technol Co Ltd, Harbin 150000, Peoples R China

4.Univ Plymouth, Sch Biol & Marine Sci, Fac Sci & Engn, Plymouth PL4 8AA, England

5.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Henan Joint Int Lab Crop Multiom Res, Kaifeng 475004, Peoples R China

6.Hainan Acad Agr Sci, Lab Mol Breeding Design, Maize Sanya Inst, Sanya 572000, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2023 年 193 卷 3 期

页码：

收录情况： SCI

摘要： Plant metaxylem vessels provide physical support to promote upright growth and the transport of water and nutrients. A detailed characterization of the molecular network controlling metaxylem development is lacking. However, knowledge of the events that regulate metaxylem development could contribute to the development of germplasm with improved yield. In this paper, we screened an EMS-induced B73 mutant library, which covers 92% of maize (Zea mays) genes, to identify drought-sensitive phenotypes. Three mutants were identified, named iqd27-1, iqd27-2, and iqd27-3, and genetic crosses showed that they were allelic to each other. The causal gene in these 3 mutants encodes the IQ domain-containing protein ZmIQD27. Our study showed that defective metaxylem vessel development likely causes the drought sensitivity and abnormal water transport phenotypes in the iqd27 mutants. ZmIQD27 was expressed in the root meristematic zone where secondary cell wall deposition is initiated, and loss-of-function iqd27 mutants exhibited a microtubular arrangement disorder. We propose that association of functional ZmIQD27 with microtubules is essential for correct targeted deposition of the building blocks for secondary cell wall development in maize.