Improving linolenic acid content in rapeseed oil by overexpression of CsFAD2 and CsFAD3 genes
文献类型： 外文期刊
作者： Liu, Yunhao 1 ; Du, Zhuolin 1 ; Li, Ying 1 ; Lu, Shaoping 1 ; Tang, Shan 1 ; Guo, Liang 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Wuhan 430070, Peoples R China
3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Shenzhen Branch,Genome Anal Lab,Minist Agr, Shenzhen 518000, Peoples R China
关键词： Rapeseed; CsFAD2; CsFAD3; Linolenic acid
期刊名称：MOLECULAR BREEDING （ 影响因子：3.1； 五年影响因子：3.1 ）
ISSN： 1380-3743
年卷期： 2024 年 44 卷 2 期
页码：
收录情况： SCI
摘要： With the increasing public attention to the health benefit of polyunsaturated fatty acids (PUFAs) and demand for linolenic acid (C18:3), it is of great significance to increase the C18:3 content in our meal. As an oil crop with high content of C18:3, Camelina sativa has three homologous copies of FAD2 and three homologous copies FAD3. In this study, we seed-specifically overexpressed two Camelina sativa fatty acid desaturase genes, CsFAD2 and CsFAD3, in rapeseed cultivar Zhongshuang 9. The results show that C18:3 content in CsFAD2 and CsFAD3 overexpressed seeds is increased from 8.62% in wild-type (WT) to 10.62-12.95% and 14.54-26.16%, respectively. We crossed CsFAD2 and CsFAD3 overexpression lines, and stable homozygous digenic crossed lines were obtained. The C18:3 content was increased from 8.62% in WT to 28.46-53.57% in crossed overexpression lines. In addition, we found that the overexpression of CsFAD2 and CsFAD3 had no effect on rapeseed growth, development, and other agronomic traits. In conclusion, we successfully generated rapeseed germplasms with high C18:3 content by simultaneously overexpressing CsFAD2 and CsFAD3, which provides a feasible way for breeding high C18:3 rapeseed cultivars.
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