Development and validation of a blocking ELISA for measurement of rabies virus neutralizing antibody
文献类型： 外文期刊
作者： Xiao, Yuewen 1 ; Wu, Meng 2 ; Xu, Weidi 1 ; Sun, Sheng 3 ; Liu, Tingfang 3 ; Liu, Yan 3 ; Li, Hongwei 4 ; Feng, Na 3 ; Tu, Changchun 1 ; Feng, Ye 3 ;
作者机构： 1.Jilin Agr Univ, Coll Anim Med, Changchun, Jilin Province, Peoples R China
2.Chongqing Acad Anim Sci, Inst Bioengn, Chongqing, Peoples R China
3.Chinese Acad Agr Sci, Changchun Vet Res Inst, State Key Lab Pathogen & Biosecur, Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun, Jilin Province, Peoples R China
4.Southern Med Univ, Sch Lab Med & Biotechnol, Guangzhou, Guangdong, Peoples R China
5.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu Provinc, Peoples R China
6.Acad Mil Med Sci, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China
关键词： rabies virus glycoprotein; monoclonal antibody; blocking ELISA; neutralizing antibody detection; validation
期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）
ISSN： 0095-1137
年卷期： 2025 年 63 卷 6 期
页码：
收录情况： SCI
摘要： Enzyme-linked immunosorbent assay (ELISA) is an effective approach for monitoring herd immunity of animals against rabies but not recommended to detect neutralizing antibody response of individual animals in the framework of international travel since its insufficient agreement with the globally recognized rabies virus (RABV) neutralizing tests. In this study, a blocking ELISA to specifically detect anti-RABV neutralizing antibody was developed using the purified RABV glycoprotein (G) expressed in HEK293T cells as coating antigen, and a labeled anti-G neutralizing monoclonal antibody as the blocking antibody. The overall agreement between the blocking ELISA and fluorescent antibody virus neutralization test in detection of clinical serum samples (dogs = 658; cats = 508) was 97.43% (1,136/1,166), with a diagnostic specificity of 95.63% (219/229) and a diagnostic sensitivity of 97.87% (917/937). Further comparison with the commercial ELISA kits and inter-laboratory validation showed that the blocking ELISA had excellent specificity, sensitivity, and reproducibility. In conclusion, the developed method is a potential tool as an alternative to the virus neutralization test for the detection of canine and feline rabies neutralization antibodies following vaccination.IMPORTANCEThis study establishes a blocking enzyme-linked immunosorbent assay (ELISA) for detecting rabies neutralizing antibodies in dogs and cats, demonstrating high sensitivity, specificity, and no cross-reactivity. This method provides a reliable alternative to conventional neutralization assays, facilitating efficient large-scale rabies vaccination assessment, and thereby strengthening global rabies control efforts.
- 相关文献
作者其他论文 更多>>
-
Structural insights into the mechanism of phosphate recognition and transport by XPR1
作者：Zhang, Wenhui;Chen, Yanke;Guan, Zeyuan;Tang, Meng;Du, Zhangmeng;Zhang, Jie;Cheng, Meng;Zuo, Jiaqi;Liu, Yan;Wang, Qiang;Liu, Yanjun;Zhang, Delin;Yin, Ping;Ma, Ling;Liu, Zhu;Wang, Yong;Liu, Zhu
关键词：
-
Yes-associated protein 1 is essential for maintaining lactation via regulating mammary epithelial cell dynamics and secretion capacity
作者：Lv, Haimiao;Wu, Zihui;Lin, Yuxin;Liu, Miaoyu;Xu, Linghua;Wang, Xiaojie;Sun, Nan;Abdelrahman, Mohamed;Yang, Liguo;Hua, Guohua;Lv, Haimiao;Hua, Guohua;Lv, Haimiao;Hua, Guohua;Xu, Xiaoling;Liu, Yan;Abdel-Shafy, Hamdy;Abdelrahman, Mohamed;Alsaegh, Aiman A.;Ahmed, Ahmed Ezzat;Ahmed, Ahmed Ezzat;Yang, Liguo;Hua, Guohua
关键词：Lactation; Yes-associated protein1(YAP1); Prolactin; Melatonin; Mammary epithelial cells
-
A compendium of 8,176 bat RNA viral metagenomes reveals ecological drivers and circulation dynamics
作者：Yan, Xiaomin;Liu, Yang;Li, Chenxi;Liu, Yuhang;Li, Nan;Sun, Yue;Yi, Le;Tu, Changchun;He, Biao;Hu, Tingsong;Zhang, Fuqiang;Huang, Zhenglanyi;Feng, Jiang;Jiang, Tinglei;Guo, Lei;Zhang, Hailin;Wu, Jianmin;Tu, Changchun
关键词：
-
A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease
作者：Yang, Wanying;Zhou, Wujie;Liang, Bo;Hu, Xiaojun;Wang, Shen;Wang, Zhenshan;Wang, Tiecheng;Xia, Xianzhu;Feng, Na;Zhao, Yongkun;Yan, Feihu;Yang, Wanying;Hu, Xiaojun;Wang, Zhenshan
关键词：Marburg virus; recombinant vesicular stomatitis virus; Syrian hamster; surrogate model; recurrence of classic symptoms; vaccine evaluation and drug screening
-
Emergence of Canine Parvovirus Type 2a and Canine Enteric Coronavirus in Diarrheal Outbreaks Among Farmed Raccoon Dogs in China
作者：Lu, Rongguang;Yang, Wei;Qian, Tingli;Li, Guobao;Lu, Rongguang;Cao, Haixu;Fu, Jianhai;Zhang, Chengqi;Hu, Bo;Xu, Liwen;Li, Shuangshuang;Zhao, Guanyu;Bai, Xue;Feng, Na;Shi, Qiumei;Zang, Deyue
关键词：epidemiology; evolution; mixed infections; molecular characteristics; receptor interaction
-
Phylogeography Analysis Reveals Rabies Epidemiology, Evolution, and Transmission in the Philippines
作者：Zhang, Liang;Jiang, Jianfeng;Gong, Wenjie;Zhang, Liang;Sun, Sheng;Liu, Yan;Tu, Changchun;Gong, Wenjie;Feng, Ye;Cruz, Jeffrey;Gonzales, Riva Marie;Azul, Rachel R.;Pena, Rainelda C. Dela;Tian, Yao;Jiang, Tao;Fang, Liqun;Feng, Ye;Wang, Yuyang;Tu, Changchun
关键词：rabies virus; Philippines; molecular epidemiology; transmission patterns; selection pressure; strategy
-
Effects of cloprostenol sodium and carbetocin on synchronous parturition and colostrum composition in large white sows
作者：Gao, Hongmei;Zhang, Longchao;Wang, Dong;Wang, Lixian;Peng, Miaolian;Zhong, Rongzhi;Xue, Zhenhua;Liu, Zhenqiang;Weng, Shiqiao;Liu, Yan;Tian, Jianhui;Tian, Jianhui;Tian, Jianhui
关键词：Cloprostenol sodium; Carbetocin; Large white sows; Farrowing induction; Colostrum
