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Anti-inflammatory actions of acetate, propionate, and butyrate in fetal mouse jejunum cultures ex vivo and immature small intestinal cells in vitro

文献类型： 外文期刊

作者： Huang, Shengnan 1 ; Gao, Yanan 1 ; Wang, Ziwei 1 ; Yang, Xue 1 ; Wang, Jiaqi 1 ; Zheng, Nan 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Milk & Dairy Prod, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Dairy Prod, Beijing, Peoples R China

3.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Milk & Dairy Prod Inspect Ctr, Beijing, Peoples R China

4.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing, Peoples R China

5.Qingdao Agr Univ, Coll Food Sci & Engn, Qingdao, Peoples R China

关键词： inflammation of immature small intestine; inflammatory cytokines; RNA-seq; short-chain fatty acids; signaling pathway

期刊名称：FOOD SCIENCE & NUTRITION （ 影响因子：3.553； 五年影响因子：3.634 ）

ISSN： 2048-7177

年卷期：

页码：

收录情况： SCI

摘要： Necrotizing enterocolitis (NEC) is an intestinal disease that frequently occurs in premature infants. Presently, there is no effective therapy for NEC. Therefore, the key to reduce the incidence rate of NEC is to take effective intervention measures as early as possible. Short-chain fatty acids (SCFAs) (acetate, propionate, and butyrate), the principal terminal products of enterobacteria fermentation, play anti-inflammatory actions in mature intestinal cells. However, few studies focus on their roles in immature intestine. Here, we evaluated the anti-inflammatory actions of SCFAs ex vivo with ICR fetal mouse jejunum cultures and explored the potential anti-inflammatory regulators through RNA-seq and then verified them in vitro with human fetal small intestinal epithelial FHs 74 Int cells. In this study, we found that acetate, propionate, and butyrate decreased IL-1 beta-induced production of CXCL2 ex vivo and IL-8 and IL-6 in vitro significantly (p < .05). Furthermore, the inhibitors of NF-kappa B p65, JNK1/2, and ERK1/2 pathways, which were selected from RNA-seq and depressed by SCFAs, also significantly decreased IL-8 and IL-6 productions induced by IL-1 beta (p < .05). Therefore, our results showed that acetate, propionate, and butyrate ameliorated the fetal small intestine inflammatory response induced by IL-1 beta through inhibiting ERK1/2 pathway; NF-kappa B p65, JNK1/2, and ERK1/2 pathways; or NF-kappa B p65 and ERK1/2 pathways, respectively. These findings suggested that SCFAs may be a new therapy agent for NEC.

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