Improvement of Flowering Stage in Japonica Rice Variety Jiahe212 by Using CRISPR/Cas9 System
文献类型： 外文期刊
作者： He, Dengmei 1 ; Zhou, Ran 2 ; Huang, Chenbo 2 ; Li, Yanhui 2 ; Peng, Zequn 2 ; Li, Dian 2 ; Duan, Wenjing 2 ; Huang, Nuan 2 ; Cao, Liyong 2 ; Cheng, Shihua 2 ; Zhan, Xiaodeng 2 ; Sun, Lianping 2 ; Wang, Shiqiang 1 ;
作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Agron, Daqing 163711, Peoples R China
2.China Natl Rice Res Inst, Chinese Natl Ctr Rice Improvement, Hangzhou 311402, Peoples R China
3.China Natl Rice Res Inst, Natl Key Lab Rice Biol & Breeding, Hangzhou 311402, Peoples R China
4.Northern Rice Res Ctr China Natl Rice Res Inst, Baoqing Northern Rice Res Ctr, Baoqing 155600, Heilongjiang, Peoples R China
5.China Natl Rice Res Inst, Fuyuan Collaborat Breeding Innovat Ctr, Jiamusi 156500, Peoples R China
6.Hechi Univ, Coll Chem & Biol Engn, Hechi 546300, Peoples R China
关键词： rice; flowering period; multiplex genome editing; breed improvement
期刊名称：PLANTS-BASEL （ 影响因子：4.0； 五年影响因子：4.4 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 15 期
页码：
收录情况： SCI
摘要： The flowering period of rice significantly impacts variety adaptability and yield formation. Properly shortening the reproductive period of rice varieties can expand their ecological range without significant yield reduction. Targeted genome editing, like CRISPR/Cas9, is an ideal tool to fine-tune rice growth stages and boost yield synergistically. In this study, we developed a CRISPR/Cas9-mediated multiplex genome-editing vector containing five genes related to three traits, Hd2, Ghd7, and DTH8 (flowering-stage genes), along with the recessive rice blast resistance gene Pi21 and the aromatic gene BADH2. This vector was introduced into the high-quality japonica rice variety in Zhejiang province, Jiahe212 (JH212), resulting in 34 T-0 plants with various effective mutations. Among the 17 mutant T-1 lines, several displayed diverse flowering dates, but most exhibited undesirable agronomic traits. Notably, three homozygous mutant lines (JH-C15, JH-C18, and JH-C31) showed slightly earlier flowering dates without significant differences in yield-related traits compared to JH212. Through special Hyg and Cas marker selection of T-2 plants, we identified seven, six, and two fragrant glutinous plants devoid of transgenic components. These single plants will serve as sib lines of JH212 and potential resources for breeding applications, including maintenance lines for indica-japonica interspecific three-line hybrid rice. In summary, our research lays the foundation for the creation of short-growth-period CMS (cytoplasmic male sterility, CMS) lines, and also provides materials and a theoretical basis for indica-japonica interspecific hybrid rice breeding with wider adaptability.
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