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The putative vacuolar processing enzyme gene TaVPE3cB is a candidate gene for wheat stem pith-thickness

文献类型： 外文期刊

作者： Liu, Qier 1 ; Zhao, Yun 1 ; Rahman, Shanjida 1 ; She, Maoyun 1 ; Zhang, Jingjuan 1 ; Yang, Rongchang 1 ; Islam, Shahidul 1 ; O'Hara, Graham 1 ; Varshney, Rajeev K. 1 ; Liu, Hang 1 ; Ma, Hongxiang 5 ; Ma, Wujun 1 ;

作者机构： 1.Murdoch Univ, Food Futures Inst, Ctr Crop & Food Innovat, Perth, WA 6150, Australia

2.Murdoch Univ, Coll Sci Hlth Engn & Educ, Perth, WA 6150, Australia

3.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Shijiazhuang 050035, Hebei, Peoples R China

4.Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China

5.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China

6.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 6 期

页码：

收录情况： SCI

摘要： Key messageThe vacuolar processing enzyme gene TaVPE3cB is identified as a candidate gene for a QTL of wheat pith-thickness on chromosome 3B by BSR-seq and differential expression analyses.The high pith-thickness (PT) of the wheat stem could greatly enhance stem mechanical strength, especially the basal internodes which support the heavier upper part, such as upper stems, leaves and spikes. A QTL for PT in wheat was previously discovered on 3BL in a double haploid population of 'Westonia' x 'Kauz'. Here, a bulked segregant RNA-seq analysis was applied to identify candidate genes and develop associated SNP markers for PT. In this study, we aimed at screening differentially expressed genes (DEGs) and SNPs in the 3BL QTL interval. Sixteen DEGs were obtained based on BSR-seq and differential expression analyses. Twenty-four high-probability SNPs in eight genes were identified by comparing the allelic polymorphism in mRNA sequences between the high PT and low PT samples. Among them, six genes were confirmed to be associated with PT by qRT-PCR and sequencing. A putative vacuolar processing enzyme gene TaVPE3cB was screened out as a potential PT candidate gene in Australian wheat 'Westonia'. A robust SNP marker associated with TaVPE3cB was developed, which can assist in the introgression of TaVPE3cB.b in wheat breeding programs. In addition, we also discussed the function of other DEGs which may be related to pith development and programmed cell death (PCD). A five-level hierarchical regulation mechanism of stem pith PCD in wheat was proposed.

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