文献类型： 外文期刊

作者： Chen, Lina ¹ ; Wang, Hao ¹ ; Xu, Tingtao ¹ ; Liu, Ruitao ¹ ; Zhu, Juanli ¹ ; Li, Haoxian ¹ ; Zhang, Huawei ⁵ ; Tang, Liying ¹ ; Jing, Dan ¹ ; Yang, Xuanwen ¹ ; Guo, Qigao ⁶ ; Wang, Peng ⁶ ; Wang, Luwei ¹ ; Liu, Junhao ¹ ; Duan, Shuyun ¹ ; Liu, Zhaoning ¹ ; Huang, Mengchi ¹ ; Li, Xiaolong ⁷ ; Lu, Zhenhua ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Zhengzhou, Peoples R China

2.Chinese Acad Agr Sci, Zhongyuan Res Ctr, Xinxiang, Peoples R China

3.Chinese Acad Agr Sci, Natl Nanfan Res Inst, Sanya, Peoples R China

4.Chinese Acad Agr Sci, Chuxiong Yunguo Agr Technol Res Inst, Chuxiong, Yunnan, Peoples R China

5.Peking Univ, Inst Adv Agr Sci, Natl Key Lab Wheat Improvement, Shandong Lab Adv Agr Sci Weifang, Weifang, Shandong, Peoples R China

6.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing, Peoples R China

7.OMIX Technol Corp, Chengdu, Peoples R China

关键词： pomegranate; T2T genome; GWAS; anthocyanin synthesis; secondary cell wall thickening; gene editing

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）

ISSN： 1467-7644

年卷期： 2025 年 23 卷 7 期

页码：

收录情况： SCI

摘要： Pomegranate is an important perennial fruit tree distributed worldwide. Reference genomes with gaps and limit gene identification controlling important agronomic traits hinder its functional genomics and genetic improvements. Here, we reported a telomere-to-telomere (T2T) gap-free genome assembly of the distinctive cultivar 'Moshiliu'. The Moshiliu reference genome was assembled into eight chromosomes without gaps, totalling similar to 366.71 Mb, with 32 158 predicted protein-coding genes. All 16 telomeres and eight centromeres were characterized; combined with FISH analysis, we revealed the atypical telomere units in pomegranate as TTTTAGGG. Furthermore, a total of 16 loci associated with 15 important agronomic traits were identified based on GWAS of 146 accessions. Gene editing and biochemical experiments demonstrated that a 37.2-Kb unique chromosome translocation disrupting the coding domain sequence of PgANS was responsible for anthocyanin-less, knockout of PgANS in pomegranate exhibited a defect in anthocyanin production; a unique repeat expansion in the promoter of PgANR may affected its expression, resulting in black peel; notably, the G -> A transversion located at the 166-bp coding domain of PgNST3, which caused a E56K mutation in the PgNST3 protein, closely linked with soft-seed trait. Overexpression of PgNST3(A) in tomato presented smaller and softer seed coats. The E56K mutation in PgNST3 protein, eliminated the binding ability of PgNST3 to the PgMYB46 promoter, which subsequently affected the thickness of the inner seed coat of soft-seeded pomegranates. Collectively, the validated gap-free genome, the identified genes controlling important traits and the CRISPR-Cas9-mediated gene knockout system all provided invaluable resources for pomegranate precise breeding.