Identification and functional characterization of a flavonol synthase gene from sweet potato [Ipomoea batatas (L.) Lam.]
文献类型： 外文期刊
作者： Kou, Meng 1 ; Li, Chen 1 ; Song, Weihan 1 ; Shen, Yifan 1 ; Tang, Wei 1 ; Zhang, Yungang 1 ; Wang, Xin 1 ; Yan, Hui 1 ; Gao, Runfei 1 ; Ahmad, Muhammad Qadir 2 ; Li, Qiang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Xuzhou Inst Agr Sci Jiangsu Xuhuai Dist, Key Lab Biol & Genet Breeding Sweet Potato, Sweet Potato Res Inst,Minist Agr & Rural Affairs, Xuzhou, Peoples R China
2.Bahauddin Zakariya Univ, Dept Plant Breeding & Genet, Multan, Pakistan
关键词： sweet potato; flavonol synthase; flavonols; anthocyanins; RNAi
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： Flavonol synthase (FLS) is a key enzyme of the flavonoid biosynthetic pathway, which catalyzes the conversion of dihydroflavonols into flavonols. In this study, the FLS gene IbFLS1 was cloned and characterized from sweet potato. The resulting IbFLS1 protein showed a high similarity with other plant FLSs. The conserved amino acids (HxDxnH motifs) binding ferrous iron and residues (RxS motifs) binding 2-oxoglutarate were found in IbFLS1 at conserved positions, as in other FLSs, suggesting that IbFLS1 belongs to the 2-oxoglutarate-dependent dioxygenases (2-ODD) superfamily. qRT-PCR analysis showed an organ-specific pattern of expression of the IbFLS1 gene, which was predominantly expressed in young leaves. The recombinant IbFLS1 protein could catalyze the conversion of dihydrokaempferol and dihydroquercetin to kaempferol and quercetin, respectively. The results of subcellular localization studies indicated that IbFLS1 was found mainly in the nucleus and cytomembrane. Furthermore, silencing the IbFLS gene in sweet potato changed the color of the leaves to purple, substantially inhibiting the expression of IbFLS1 and upregulating the expression of genes involved in the downstream pathway of anthocyanin biosynthesis (i.e., DFR, ANS, and UFGT). The total anthocyanin content in the leaves of the transgenic plants was dramatically increased, whereas the total flavonol content was significantly reduced. Thus, we conclude that IbFLS1 is involved in the flavonol biosynthetic pathway and is a potential candidate gene of color modification in sweet potato.
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