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ISOLATION AND CHARACTERIZATION OF THE LIPOXYGENASE-3 PROMOTER IN NICOTIANA TABACUM

文献类型： 外文期刊

作者： Xie, Minmin 1 ; Li, Shanshan 4 ; Li, Zhiyuan 1 ; Chen, Mingli 1 ; Gong, Daping 1 ; Richel, Aurore 3 ;

作者机构： 1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao 266101, Peoples R China

2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China

3.Univ Liege, Lab Biomass & Green Technol, Gembloux Agrobio Tech, B-5030 Gembloux, Belgium

4.Yunnan Minzu Univ, Key Lab Nat Prod Synthet Biol Ethn Med, State Ethn Affairs Commiss, Kunming, Peoples R China

关键词： Lipoxygenase; Wounding; JA; Transgenic plants.

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：1.2； 五年影响因子：1.1 ）

ISSN： 0556-3321

年卷期： 2024 年 56 卷 1 期

页码：

收录情况： SCI

摘要： The jasmonates (JA) play key roles in defending mechanical wounding, herbivore attack and various stresses in plants. Lipoxygenase (LOX) is one of the key enzymes in the biosynthesis of JA which also catalyzes the green leafy volatiles synthesis at the first step. Wounding and herbivore attack can induce the expression of LOXs. To investigate the molecular mechanism regulating the LOX genes in tobacco, we isolated and identified the LOX3 gene promoter from Nicotiana tabacum. Analysis from transgenic plants and GUS activity fluorimetric assay revealed that 1847bp of NtLOX3 promoter sequence was sufficient for wounding and methyl jasmonic acid (MeJA) induction. For wounding and MeJA induction, the deletion analysis identified the minimal effective regions which were located in -546bp to -199bp. And this region contained the cis-acting elements of CATG-box (CATG), W-box (C/TTGACY) and a T/G-box (AACGTG), which were thought to play a key role in wounding and MeJA elicitation. These results may provide information for further transcriptional regulation studies of LOX genes.

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