文献类型： 外文期刊

作者： Chu, Bo ¹ ; Ge, Shishuai ² ; He, Wei ² ; Sun, Xiaoting ² ; Ma, Jiajie ² ; Yang, Xianming ² ; Lv, Chunyang ² ; Xu, Pengjun ⁷ ; Zhao, Xincheng ¹ ; Wu, Kongming ² ;

作者机构： 1.Henan Agr Univ, Coll Plant Protect, Henan Int Joint Lab Green Pest Control, Zhengzhou 450002, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

3.Henan Inst Sci & Technol, Sch Resources & Environm, Xinxiang 453003, Peoples R China

4.Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou 350002, Peoples R China

5.Hainan Univ, Coll Trop Crops, Haikou 570228, Peoples R China

6.Henan Univ, Coll Agr, Sch Life Sci, State Key Lab Cotton Biobreeding & Integrated Util, Kaifeng 475004, Peoples R China

7.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Pest Monitoring Controlling & Inte, Qingdao 266101, Peoples R China

关键词： Fall armyworm; Gut bacteria; Juvenile hormone III; 20-hydroxyecdysone; Fecundity

期刊名称：MICROBIOME （ 影响因子：12.7； 五年影响因子：16.6 ）

ISSN： 2049-2618

年卷期： 2025 年 13 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe insect gut microbiota forms a complex, multifunctional system that significantly affects phenotypic traits linked to environmental adaptation. Strong reproductive potential underpins the migratory success, population growth and destructive impact of the fall armyworm, Spodoptera frugiperda (J.E. Smith). However, the precise role of gut bacteria in S. frugiperda reproductive processes, distribution and transmission dynamics remains unclear.ResultsWe examined the gut microbiota of S. frugiperda a major invasive agricultural pest, identifying Enterococcus, Enterobacter, and Klebsiella as core microorganisms present throughout its life cycle. These microbes showed heightened activity during the egg stage, early larval stages and pre-oviposition period in females. Using an axenic insect re-infection system, Enterococcus quebecensis FAW181, Klebsiella michiganensis FAW071 and Enterobacter hormaechei FAW049 were found to significantly enhance host fecundity, increasing egg production by 62.73%, 59.95%, and 56.71%, respectively. Metagenomic and haemolymph metabolomic analyses revealed a positive correlation between gut symbiotic bacteria and hormone metabolism in female S. frugiperda. Further analysis of metabolites in the insect hormone biosynthesis pathway, along with exogenous injection of juvenile hormone III and 20-hydroxyecdysone, revealed that gut microbes regulate these hormones, maintaining levels equivalent to those in control insects. This regulation supports improved fecundity in S. frugiperda, aiding rapid colonization and population expansion.ConclusionsThese findings emphasize the pivotal role of gut bacteria E. quebecensis FAW181, E. hormaechei FAW049, and K. michiganensis FAW071 in enhancing S. frugiperda reproduction by modulating JH III levels through JHAMT regulation and concurrently modulating the levels of 20E and its precursors via PHM. Our results provide novel insights into microbe-host symbiosis and pest management strategies for alien invasive species.77wuTZZCu29-t85L67975jVideo AbstractConclusionsThese findings emphasize the pivotal role of gut bacteria E. quebecensis FAW181, E. hormaechei FAW049, and K. michiganensis FAW071 in enhancing S. frugiperda reproduction by modulating JH III levels through JHAMT regulation and concurrently modulating the levels of 20E and its precursors via PHM. Our results provide novel insights into microbe-host symbiosis and pest management strategies for alien invasive species.77wuTZZCu29-t85L67975jVideo Abstract