Functional Characterization of 5-O-Glycosyltranferase Transforming 3-O Anthocyanins into 3,5-O Anthocyanins in Freesia hybrida
文献类型： 外文期刊
作者： Adnan 1 ; Bao, Tingting 1 ; Zheng, Xiang 1 ; Pang, Yicong 1 ; Gao, Ruifang 1 ; Shan, Xiaotong 1 ; Zhu, Shirui 1 ; Kimani, Shadrack Kanyonji 1 ; Gao, Xiang 1 ; Li, Yueqing 1 ;
作者机构： 1.Northeast Normal Univ, Key Lab Mol Epigenet MOE, Changchun 130024, Peoples R China
2.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Synthet Biol, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,, Shenzhen 518120, Peoples R China
关键词： anthocyanidin; flavonoid; flower color; modification genes; MBW complex
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 10 期
页码：
收录情况： SCI
摘要： Floral coloration in Freesia hybrida is predominantly attributed to anthocyanins, with glycosylation playing a critical role in their stability and diversity. This study investigates the molecular mechanisms underlying color variation between F. hybrida cultivars, focusing on anthocyanin 5-O-glucosyltransferases (An5GTs). HPLC analysis revealed that 'Pink Passion' petals accumulate 3,5-O-diglucosidic anthocyanins, absent in 'Red River (R)' and 'Ambiance'. RNA-seq identified seven candidate Fh5GT genes, with phylogenetic and subcellular localization analyses confirming their classification as cytosolic glycosyltransferases. Expression profiling highlighted elevated transcript levels of Fh5GT1, Fh5GT3, and Fh5GT7 in 'Pink Passion', correlating with its di-glucosidic anthocyanin accumulation. In vitro enzymatic assays demonstrated that Fh5GT3 and Fh5GT7 preferentially glucosylate 3-O-monoglucosidic anthocyanins to form stable 3,5-O-diglucosides, with minimal activity on anthocyanidins to generate 5-O-glucosidic anthocyanins. Heterologous expression of Fh5GT3 and Fh5GT7 in Arabidopsis complemented anthocyanin deficiency in 5gt mutants, restoring pigmentation. These findings elucidate the potential role of 5GTs in modulating floral color diversity through anthocyanin modification, providing insights for targeted breeding strategies to enhance ornamental traits in horticultural species.
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