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The nematode effector calreticulin competes with the high mobility group protein OsHMGB1 for binding to the rice calmodulin-like protein OsCML31 to enhance rice susceptibility to Meloidogyne graminicola

文献类型： 外文期刊

作者： Liu, Jing 1 ; Zhang, Jiaqian 1 ; Wei, Ying 1 ; Su, Wen 1 ; Li, Wei 1 ; Wang, Bing 1 ; Peng, Deliang 3 ; Gheysen, Godelieve 2 ; Peng, Huan 3 ; Dai, Liangying 1 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Key Lab Biol & Control Plant Dis & Insect Pests, Changsha, Hunan, Peoples R China

2.Univ Ghent, Dept Mol Biotechnol, Ghent, Belgium

3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China

4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

5.Hunan Agr Univ, Coll Plant Protect, Key Lab Biol & Control Plant Dis & Insect Pests, Changsha 410128, Hunan, Peoples R China

关键词： calmodulin-like protein; calreticulin; effector; Meloidogyne graminicola; OsHMGB1; plant defense

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.3； 五年影响因子：8.1 ）

ISSN： 0140-7791

年卷期： 2024 年 47 卷 5 期

页码：

收录情况： SCI

摘要： The root-knot nematode Meloidogyne graminicola secretes effectors into rice tissues to modulate host immunity. Here, we characterised MgCRT1, a calreticulin protein of M. graminicola, and identified its target in the plant. In situ hybridisation showed MgCRT1 mRNA accumulating in the subventral oesophageal gland in J2 nematodes. Immunolocalization indicated MgCRT1 localises in the giant cells during parasitism. Host-induced gene silencing of MgCRT1 reduced the infection ability of M. graminicola, while over-expressing MgCRT1 enhanced rice susceptibility to M. graminicola. A yeast two-hybrid approach identified the calmodulin-like protein OsCML31 as an interactor of MgCRT1. OsCML31 interacts with the high mobility group protein OsHMGB1 which is a conserved DNA binding protein. Knockout of OsCML31 or overexpression of OsHMGB1 in rice results in enhanced susceptibility to M. graminicola. In contrast, overexpression of OsCML31 or knockout of OsHMGB1 in rice decreases susceptibility to M. graminicola. The GST-pulldown and luciferase complementation imaging assay showed that MgCRT1 decreases the interaction of OsCML31 and OsHMGB1 in a competitive manner. In conclusion, when M. graminicola infects rice and secretes MgCRT1 into rice, MgCRT1 interacts with OsCML31 and decreases the association of OsCML31 with OsHMGB1, resulting in the release of OsHMGB1 to enhance rice susceptibility.

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