Involvement of CYP347W1 in neurotoxin 3-nitropropionic acid-based chemical defense in mustard leaf beetle Phaedon cochleariae
文献类型： 外文期刊
作者： Fu, Nanxia 1 ; Becker, Tobias 1 ; Brandt, Wolfgang 3 ; Kunert, Maritta 1 ; Burse, Antje 1 ; Boland, Wilhelm 1 ;
作者机构： 1.Max Planck Inst Chem Ecol, Dept Bioorgan Chem, Hans Knoll Str 8, D-07745 Jena, Germany
2.Chinese Acad Agr Sci, Tea Res Inst, Key Lab Tea Biol & Resource Utilizat, Hangzhou, Peoples R China
3.Leibniz Inst Plant Biochem, Dept Bioorgan Chem, Halle, Saale, Germany
4.Max Planck Inst Chem Ecol, Dept Nat Prod Biosynth, Jena, Germany
5.Ernst Abbe Hsch Jena, Dept Med Technol & Biotechnol, Jena, Germany
关键词： chemical defense; isoxazolin-5-one glucoside; isoxazolin-5-one glucoside ester; leaf beetles; P450; 3-nitropropionic acid (3-NPA)
期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）
ISSN： 1672-9609
年卷期：
页码：
收录情况： SCI
摘要： Chrysomelina beetlesstore 3-nitropropionic acid in form of a pretoxin, isoxazolin-5-one glucoside-conjugated ester, to protect themselves against predators. Here we identified a cytochrome P450 monooxygenase, CYP347W1, to be involved in the production of the 3-nitropropionic acid moiety of the isoxazolin-5-one glucoside ester. Knocking down CYP347W1 led to a significant depletion in the concentration of the isoxazolin-5-one glucoside ester and an increase in the concentration of the isoxazolin-5-one glucoside in the larval hemolymph. Enzyme assays with the heterologously expressed CYP347W1 showed free beta-alanine was not the direct substrate. Homology modeling indicated that beta-alanine-CoA ester can fit into CYP347W1's active site. Furthermore, we proved that Phaedon cochleariae eggs are not able to de novo synthesize 3-NPA, although both isoxazolin-5-one glucoside and its 3-NPA-conjugated ester are present in the eggs. These results provide direct evidence for the involvement of CYP347W1 in the biosynthesis of a P. cochleariae chemical defense compound.
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