文献类型： 外文期刊

作者： Fu, Yu ¹ ; Chen, Yupeng ² ; Wang, Yani ¹ ; Fu, Xinyi ¹ ; Jin, Shunda ⁴ ; Yi, Chunyan ⁶ ; Bai, Xue ⁷ ; Lu, Youqing ¹ ; Miao, Wang ¹ ; Geng, Xingyu ¹ ; Lu, Xianli ¹ ; Yan, Rihui ¹ ; Zhou, Zhongshi ⁸ ; Cao, Fengqin ¹ ;

作者机构： 1.Hainan Univ, Sch Trop Agr & Forestry, Haikou 570100, Peoples R China

2.Hong Kong Baptist Univ, Inst Syst Med & Hlth Sci, Hong Kong 999077, Peoples R China

3.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong 999077, Peoples R China

4.Sanya Inst Henan Univ, Sch Life Sci, Sanya 572025, Peoples R China

5.Henan Univ, Sch Life Sci, Natl Key Lab Cotton Biobreeding & Integrated Utili, Henan Joint Int Lab Crop Multiom Res, Kaifeng 475000, Peoples R China

6.Sichuan Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Southwest Crops, Chengdu 610066, Peoples R China

7.Zhejiang Univ, State Key Lab Rice Biol & Breeding, Inst Insect Sci,Key Lab Biol Crop Pathogens & Inse, Minist Agr & Rural Affairs,Key Lab Mol Biol Crop P, Hangzhou 310058, Peoples R China

8.Chinese Acad Agr Sci, Natl Nanfan Res Inst, Sanya 572019, Peoples R China

关键词： Zeugodacus cucuribitae (Coquillett); olfactory genes; network pharmacology; molecular docking; insect repellent

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 14 期

页码：

收录情况： SCI

摘要： Zeugodacus cucuribitae (Coquillett) (Z. cucuribitae) is a global extremely invasive quarantine pest which has a wide host range of fruits and vegetables. At present, there are a few control measures for Z. cucuribitae, and deltamethrin and avermectin are commonly used. Among the hosts of Z. cucuribitae, Luffa acutangular, Luffa cylindrica, Sechium edule, Brassica oleracea var. botrytis, Musa nana, and Fragaria x ananassa are non-favored hosts. However, it is still not clear why these hosts are non-favored and whether there are any repellent components of Z. cucuribitae in these hosts. In this study, the components of these six hosts were collected from the literature, and the genes of odor and chemical sensation were determined from the genome of Z. cucuribitae. After the potential relationships between these components and genes were determined by molecular docking methods, the KEGG and GO enrichment analysis of these genes was conducted, and a complex network of genes vs. components vs. Kegg pathway vs. GO terms was constructed and used to select the key components for experiments. The results show that oleanolic acid (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), rotenone (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), and beta-caryophyllene oxide (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL) had a significant repellent effect on Z. cucuribitae, and three components, rotenone (1 mg/mL and 0.1 mg/mL), echinocystic acid (1 mg/mL, 0.1 mg/mL, and 0.01 mg/mL), and beta-caryophyllene oxide (1 mg/mL, and 0.1 mg/mL) had significant stomach toxicity in Z. cucuribitae. Furthermore, a complex signaling pathway was built and used to predict the effect of these components on Z. cucuribitae. These components probably play roles in the neuroactive ligand-receptor interaction (ko04080) and calcium signaling (ko04020) pathways. This study provides a reference for the prevention and control of Z. cucuribitae and a scientific reference for the rapid screening and development of new pest control drugs.