Anti-Inflammatory, Antioxidant, and Antifibrotic Effects of Gingival-Derived MSCs on Bleomycin-Induced Pulmonary Fibrosis in Mice
文献类型： 外文期刊
作者： Wang, Xishuai 1 ; Zhao, Shiyu 1 ; Lai, Junhui 1 ; Guan, Weijun 1 ; Gao, Yang 3 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Dept Anim Genet Resources, Beijing 100193, Peoples R China
2.Beijing Normal Univ, Coll PE & Sport, Beijing 100193, Peoples R China
3.Capital Univ Phys Educ & Sports, Inst Phys Educ Training, Beijing 100193, Peoples R China
关键词： lung fibrosis; mesenchymal stem cells; neutrophils; inflammation; oxidative stress
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 1 期
页码：
收录情况： SCI
摘要： Background: Mesenchymal stem cell (MSC) intervention has been associated with lung protection. We attempted to determine whether mouse gingival-derived mesenchymal stem cells (GMSCs) could protect against bleomycin-induced pulmonary fibrosis. Methods: Mice were divided into three groups: control (Con), bleomycin (Bl), and bleomycin + MSCs (Bl + MSCs). Mice were treated with 5 mg/kg bleomycin via transtracheal instillation to induce pulmonary fibrosis. We assessed the following parameters: histopathological severity of injury in the lung, liver, kidney, and aortic tissues; the degree of pulmonary fibrosis; pulmonary inflammation; pulmonary oedema; profibrotic factor levels in bronchoalveolar lavage fluid (BALF) and lung tissue; oxidative stress-related indicators and apoptotic index in lung tissue; and gene expression levels of IL-1 beta, IL-8, TNF-alpha, lysophosphatidic acid (LPA), lysophosphatidic acid receptor 1 (LPA1), TGF-beta, matrix metalloproteinase 9 (MMP-9), neutrophil elastase (NE), MPO, and IL-10 in lung tissue. Results: GMSC intervention attenuated bleomycin-induced pulmonary fibrosis, pulmonary inflammation, pulmonary oedema, and apoptosis. Bleomycin instillation notably increased expression levels of the IL-1 beta, IL-8, TNF-alpha, LPA, LPA1, TGF-beta, MMP-9, NE, and MPO genes and attenuated expression levels of the IL-10 gene in lung tissue, and these effects were reversed by GMSC intervention. Bleomycin instillation notably upregulated MDA and MPO levels and downregulated GSH and SOD levels in lung tissue, and these effects were reversed by GMSC intervention. GMSC intervention prevented upregulation of neutrophil content in the lung, liver, and kidney tissues and the apoptotic index in lung tissue. Conclusions: GMSC intervention exhibits anti-inflammatory and antioxidant capacities. Deleterious accumulation of neutrophils, which is reduced by GMSC intervention, is a key component of bleomycin-induced pulmonary fibrosis. GMSC intervention impairs bleomycin-induced NE, MMP-9, LPA, APL1, and TGF-beta release.
- 相关文献
作者其他论文 更多>>
-
Evaluating the Sustainability of Wheat-Maize System with a Long-Term Fertilization Experiment
作者：Shao, Yun;An, Jiahui;Wang, Xueping;Ma, Shouchen;Meng, Ye;Gao, Yang;Ma, Shoutian
关键词：long-term fertilization; soil quality; wheat and maize cropping system; sustainability index
-
Post-anthesis water use and biomass accumulation in winter wheat under subsoiling and microsprinkler irrigation
作者：Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Gao, Yang;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Gao, Yang;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Yanyan;Liu, Xuchen;Ma, Shoutian;Qin, Anzhen;Li, Ying;Liu, Zhandong;Huang, Chao;Zhang, Qifeng;Gao, Zile;Wu, Guanghui;Wang, Kai
关键词：Microsprinkler irrigation; Winter wheat; Water consumption characteristics; Post-anthesis biomass accumulation; Grain-filling characteristics; North China Plain
-
Prevalence and characterization of Cryptosporidium in sika deer from Northern China
作者：Tang, Yan;Liu, Jing;Tang, Yan;Xue, Nian-Yu;Gao, Yang;Gao, Zhen-Qiu;Cao, Hong-Wei;Zhuang, Hong-Di;Li, Jian-Ming;Bao, Guang-Rong;Liu, Shuo
关键词：Cryptosporidium sp.; Sika deer; Prevalence; Subtype; China
-
Optimization of Irrigation Parameters of Peanut Under Mulched Drip Irrigation in Xinjiang Based on Yield and Water Use Efficiency
作者：Zhang, Yuchao;Li, Shaofei;Li, Haiming;Chen, Junwei;Dong, Jianshu;Shen, Xiaojun;Zhang, Yuchao;Gao, Yang;Si, Zhuanyun;Li, Haiming;Chen, Junwei;Dong, Jianshu;Shen, Xiaojun;Cui, Weimin;Li, Qiang;Zhang, Xiaopei
关键词：drip discharge; peanut; yield; quality; water use efficiency; nitrogen partial factor productivity
-
Optimizing a water-saving and salt-controlling irrigation strategy for improving cotton yield with the AquaCrop model under mulched drip irrigation in saline-alkali soil
作者：Jiang, Fuchang;Gao, Yang;Wang, Hongbo;Wang, Xingpeng;Jiang, Fuchang;Gao, Yang;Wang, Hongbo;Wang, Xingpeng;Li, Na;Liang, Jiaping;Li, Na;Liang, Jiaping;Wang, Xingpeng;Gao, Yang;Du, Jiangtao
关键词：
-
Diagnosis and comprehensive evaluation of nitrogen nutrition in non-mulched drip irrigation cotton in southern Xinjiang based on critical nitrogen concentration
作者：Li, Guohui;Wang, Hongbo;Tang, Maosong;Wang, Xingpeng;Wang, Hongbo;Wang, Xingpeng;Wang, Hongbo;Wang, Xingpeng;Gao, Yang;Fu, Yuanyuan;Huang, Weixiong;Wang, Xingpeng
关键词：Cotton; Water and N transport; Yield; Critical dilution curve; TOPSIS
-
Integrating UAV-Based Multispectral Data and Transfer Learning for Soil Moisture Prediction in the Black Soil Region of Northeast China
作者：Zhou, Tong;Ma, Shoutian;Liu, Tianyu;Li, Shenglin;Gao, Yang;Yao, Shuihong
关键词：UAV; remote sensing; transfer learning; soil moisture; cross-region
