Multi-Omics Analysis Provides Insights into a Mosaic-Leaf Phenotype of Astaxanthin-Producing Tobacco
文献类型： 外文期刊
作者： Wang, Jialin 1 ; Du, Zaifeng 1 ; Lin, Xiaoyang 1 ; Li, Peng 2 ; Sun, Shihao 2 ; Yang, Changqing 1 ; Chen, Yong 2 ; Zhang, Zhongfeng 1 ; Yin, Xue 1 ; Fang, Ning 1 ;
作者机构： 1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Synthet Biol Minist Agr & Rural Affairs, Qingdao 266101, Peoples R China
2.Beijing Life Sci Acad BLSA, Dept Appl Res 2, Beijing 102209, Peoples R China
关键词： astaxanthin; mosaic-like spots; chlorophyll metabolism; metabolomics; proteomics; small RNA transcriptomics
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 6 期
页码：
收录情况： SCI
摘要： In metabolically engineered plants, the target products are usually uniformly distributed in the whole plant or specific tissues. When engineering tobacco to produce astaxanthin, a ketocarotenoid with strong antioxidant activity and multiple bioactivities, a scattered distribution of astaxanthin-producing regions was observed in a small portion of astaxanthin-producing tobacco plants, which caused mosaic-like red and green spots on the leaves (ASTA-mosaic). A physiological assay showed that the non-astaxanthin green region (Mosaic_G) had relatively higher chlorophyll content and better chloroplast structure than the astaxanthin-producing red region (Mosaic_R). Then, metabolomics, proteomics, and small RNA transcriptomics were employed to analyze the uneven distribution of astaxanthin-producing regions in tobacco leaves. The results of metabolomics and proteomics revealed a decrease in carotenoid metabolism, chlorophyll biosynthesis, and chlorophyll degradation in the Mosaic_G region. Pheophorbide a, an intermediate of chlorophyll degradation, was found to be significantly reduced in the Mosaic_G region, which was accompanied by the attenuation of chlorophyllase and pheophytinase, which catalyze the formation of pheophorbide a in chlorophyll degradation. Reductions in photosynthetic antenna proteins and photosystem-associated proteins were observed in the Mosaic_R region, consistent with the better chloroplast structure of the Mosaic_G region. Small RNA transcriptomics showed that several small RNAs could target chlorophyll-degradative genes, but they were more effective in targeting the astaxanthin biosynthetic genes. This finding was supported by the fact that the Mosaic_G region can remain green up to the senescence of tobacco leaves. This work provides insights into the mechanism of the uneven distribution of astaxanthin-producing regions in tobacco leaves and may contribute to the specialized utilization of tobacco plants for metabolic engineering.
- 相关文献
作者其他论文 更多>>
-
Engineering tobacco for efficient astaxanthin production using a linker-free monocistronic dual-protein expression system and interspecific hybridization method
作者：Fang, Ning;Du, Zaifeng;Liu, Xiaofeng;Tian, Tian;Wang, Wenjing;Du, Yongmei;Zhang, Zhongfeng;Zhang, Hongbo;Chai, Maofeng;Zhao, Shancen;Timko, Michael P.;Xue, Zheyong
关键词：Metabolic engineering; Astaxanthin; Monocistronic cassette; Protein splicing; Tobacco
-
How thawing and salting affects the post-cooked quality of frozen beef: New insights into the mechanism of fiber morphology evolution and water migration
作者：Lin, Hengxun;Chen, Yong;Yang, Yiping;Cui, Liye;Wu, Guangyu;Chisoro, Prince;Li, Xia;Zhang, Chunhui;Lin, Hengxun;Blecker, Christophe;Chen, Xiangning;Chen, Xiangning;Zhang, Chunhui
关键词：Freeze-induced damage; Ice crystal; Heat-induced constriction; Tissue morphology; Water migration
-
Effect of salt-induced supercooling stability on the quality of chicken breast during supercooled storage
作者：Yang, Yiping;Li, Hongliang;Huang, Junrong;Yang, Yiping;Lin, Hengxun;Chen, Yong;Cui, Liye;Xia, Shuangmei;Zhang, Chunhui;Li, Xia;Zhang, Hui;He, Jiansheng;Sun, Xuemei
关键词：Meat preservation; Salt; Supercooling stability; Freezing damage; Quality assessment
-
Talaromyces purpureogenus CEF642N as a Promising Biocontrol Agent for Cotton Disease Control
作者：Li, Peng;Wei, Feng;Feng, Hongjie;Zhao, Lihong;Zhang, Yalin;Zhou, Jinglong;Feng, Zili;Zhu, Heqin;Wei, Feng;Feng, Hongjie;Zhao, Lihong;Zhang, Yalin;Zhou, Jinglong;Feng, Zili;Zhu, Heqin
关键词：
Talaromyces purpureogenus CEF642(N); antifungal activity; secondarymetabolites; volatile organic compounds; mycoparasitism -
Identification of ZmSNAC06, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in Arabidopsis
作者：Wang, Fei;Yang, Ruisi;Luo, Ping;Wang, Houwen;Zhang, Runze;Li, Wenzhe;Yang, Ke;Xu, Xinlong;Hao, Zhuanfang;Li, Xinhai;Chen, Yong
关键词：maize (
Zea mays L.);ZmSNAC06 ; transcription factor; drought tolerance -
Ultrasound-assisted immersion freezing method as a new strategy to improve the quality and microstructure of Chinese beef stewed dishes
作者：Cui, Liye;Lin, Hengxun;Hu, Xiaojia;Li, Xia;Jia, Wei;Yang, Yiping;Chen, Yong;Zhang, Chunhui;Cui, Liye;Guan, Wenqiang
关键词：Prepared dish; Braised beef with potato; Ultrasound-assisted immersion freezing; Microstructure; Meat quality
-
Adaptation mechanisms of Brucella abortus to low magnesium ion stress
作者：Wang, Hengtai;Lv, Lang;Huang, Yike;Jiang, Hui;Yang, Xiaowen;Ding, Jiabo;Li, Peng;Zhu, Liangquan;Xu, Lei;Wang, Nan;Sang, Huaiming;Jiang, Jianxia
关键词：Brucella abortus; Low Mg2+; Transcriptome analysis; MgtC; HP3
