Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum
文献类型： 外文期刊
作者： Li, Bingjie 1 ; Chen, Chen 1 ; Cui, Mengmeng 1 ; Sun, Yuhe 1 ; Lv, Jing 1 ; Dai, Changbo 1 ;
作者机构： 1.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao, Peoples R China
2.Chinese Acad Agr Sci, Grad Sch, Beijing, Peoples R China
关键词： glyphosate; herbicide resistance; EPSP synthase; tobacco; gene editing
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2025 年 16 卷
页码：
收录情况： SCI
摘要： Glyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, the emergence of glyphosate-resistant weeds has driven the need for enhanced herbicide resistance in crops. In this study, we engineered two mutant variants of the tobacco EPSPS gene through amino acid substitution (TIPS-NtEPSPS and P180S-NtEPSPS). These mutated EPSPS genes were overexpressed in tobacco under the control of CaMV35S promoters. Our results demonstrate that overexpression of TIPS-NtEPSPS significantly enhances glyphosate tolerance, allowing plants to withstand up to four times the recommended dose without compromising their fitness. This research highlights the potential of the TIPS-NtEPSPS mutant to improve herbicide resistance in tobacco, offering a viable approach for effective weed management.
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