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Molecular traits of MAPK kinases and the regulatory mechanism of GhMAPKK5 alleviating drought/salt stress in cotton

文献类型： 外文期刊

作者： Ding, Rui 1 ; Li, Junhua 2 ; Wang, Jie 3 ; Li, Yan 1 ; Ye, Wuwei 1 ; Yan, Gentu 1 ; Yin, Zujun 1 ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biobreeding & Integrated Uti, Zhengzhou Res Base, Zhengzhou 450001, Peoples R China

2.Xinjiang Tarim River Seed Ind Co Ltd, Urumqi 518120, Xinjiang, Peoples R China

3.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biobreeding & Integrated Uti, Anyang 455000, Peoples R China

4.Chinese Acad Agr Sci, Western Agr Res Ctr, Changji 831100, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.9； 五年影响因子：7.7 ）

ISSN： 0032-0889

年卷期： 2024 年 196 卷 3 期

页码：

收录情况： SCI

摘要： Mitogen-activated protein kinase kinases (MAPKKs) play a critical role in the mitogen-activated protein kinase (MAPK) signaling pathway, transducing external stimuli into intracellular responses and enabling plant adaptation to environmental challenges. Most research has focused on the model plant Arabidopsis (Arabidopsis thaliana). The systematic analysis and characterization of MAPKK genes across different plant species, particularly in cotton (Gossypium hirsutum), are somewhat limited. Here, we identified MAPKK family members from 66 different species, which clustered into five different sub-groups, and MAPKKs from four cotton species clustered together. Through further bioinformatic and expression analyses, GhMAPKK5 was identified as the most responsive MAPKK member to salt and drought stress among the 23 MAPKKs identified in Gossypium hirsutum. Silencing GhMAPKK5 in cotton through virus-induced gene silencing (VIGS) led to quicker wilting under salt and drought conditions, while overexpressing GhMAPKK5 in Arabidopsis enhanced root growth and seed germination under these stresses, demonstrating GhMAPKK5's positive role in stress tolerance. Transcriptomics and Yeast-Two-Hybrid assays revealed a MAPK cascade signal module comprising GhMEKK (mitogen-activated protein kinase kinase kinases)3/8/31-GhMAPKK5-GhMAPK11/23. This signaling cascade may play a role in managing drought and salt stress by regulating transcription factor genes, such as WRKYs, which are involved in the biosynthesis and transport pathways of ABA, proline, and RALF. This study is highly important for further understanding the regulatory mechanism of MAPKK in cotton, contributing to its stress tolerance and offering potential in targets for genetic enhancement. Genome-wide identification and transcriptomic analyses indicate that a mitogen-activated protein kinase kinase signaling cascade plays a role in managing drought and salt stress in cotton.

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