文献类型： 外文期刊

作者： Shi, Chuanlin ¹ ; Zou, Wenli ¹ ; Zhu, Yiwang ¹ ; Zhang, Jie ² ; Teng, Chong ¹ ; Wei, Hua ¹ ; He, Huiying ¹ ; He, Wenchuang ¹ ; Liu, Xiangpei ¹ ; Zhang, Bin ¹ ; Zhang, Hong ¹ ; Leng, Yue ¹ ; Guo, Mingliang ¹ ; Wang, Xianmeng ¹ ; Chen, Wu ¹ ; Zhang, Zhipeng ¹ ; Qian, Hongge ¹ ; Cui, Yan ¹ ; Jiang, Hongshuang ¹ ; Chen, Ying ¹ ; Fei, Qili ¹ ; Meyers, Blake C. ³ ; Liang, Wanqi ² ; Qian, Qian ¹ ; Shang, Lianguang ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Genome Anal Lab, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,M, Shenzhen 518120, Peoples R China

2.Shanghai Jiao Tong Univ, Shanghai Jiao Tong Univ Univ Adelaide Joint Ctr Ag, State Key Lab Hybrid Rice, Joint Int Res Lab Metab & Dev Sci,Sch Life Sci & B, Shanghai 200240, Peoples R China

3.Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA

4.Univ Missouri, Div Plant Sci & Technol, Columbiaville, MI 65211 USA

5.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China

6.Yazhouwan Natl Lab, 8 Huanjin Rd, Sanya City 572024, Hainan, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2024 年 194 卷 4 期

页码：

收录情况： SCI

摘要： Temperature-sensitive male sterility is one of the core components for hybrid rice (Oryza sativa) breeding based on the 2-line system. We previously found that knockout of ARGONAUTE 1d (AGO1d) causes temperature-sensitive male sterility in rice by influencing phased small interfering RNA (phasiRNA) biogenesis and function. However, the specific phasiRNAs and their targets underlying the temperature-sensitive male sterility in the ago1d mutant remain unknown. Here, we demonstrate that the ago1d mutant displays normal female fertility but complete male sterility at low temperature. Through a multiomics analysis of small RNA (sRNA), degradome, and transcriptome, we found that 21-nt phasiRNAs account for the greatest proportion of the 21-nt sRNA species in rice anthers and are sensitive to low temperature and markedly downregulated in the ago1d mutant. Moreover, we found that 21-nt phasiRNAs are essential for the mRNA cleavage of a set of fertility- and cold tolerance-associated genes, such as Earlier Degraded Tapetum 1 (EDT1), Tapetum Degeneration Retardation (TDR), OsPCF5, and OsTCP21, directly or indirectly determined by AGO1d-mediated gene silencing. The loss of function of 21-nt phasiRNAs can result in upregulation of their targets and causes varying degrees of defects in male fertility and grain setting. Our results highlight the essential functions of 21-nt phasiRNAs in temperature-sensitive male sterility in rice and suggest their promising application in 2-line hybrid rice breeding in the future. Multiomics analysis of small RNAs, degradome, and transcriptome reveals 21-nucleotide phasiRNAs are essential for mRNA cleavage of genes for fertility and cold tolerance to determine temperature-sensitive male sterility in rice.