文献类型： 外文期刊

作者： Li, Xingang ¹ ; Pang, Yuanting ⁴ ; Zhong, Yiwang ¹ ; Cai, Zhandong ¹ ; Ma, Qibin ¹ ; Wen, Ke ¹ ; Nian, Hai ¹ ;

作者机构： 1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Peoples R China

2.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China

3.South China Agr Univ, Coll Agr, Natl Ctr Soybean Improvement, Guangdong Subctr, Guangzhou 510642, Peoples R China

4.Hainan Acad Agr Sci, Vegetable Res Inst, Key Lab Vegetable Biol Hainan Prov, Haikou 570228, Peoples R China

5.China Agr Univ, Coll Hort, Dept Vegetable Sci, Beijing Key Lab Growth & Dev Regulat Protected Veg, Beijing 100193, Peoples R China

6.Hainan Univ, Sanya Nanfan Res Inst, Hainan Yazhou Bay Seed Lab, Sanya 572025, Peoples R China

关键词： soybean; glutathione transferase; salt stress; glutathione

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 6 期

页码：

收录情况： SCI

摘要： Salt stress has a detrimental impact on crop yield, quality, and profitability. The tau-like glutathione transferases (GSTs) represent a significant group of enzymes that play a crucial role in plant stress responses, including salt stress. In this study, we identified a tau-like glutathione transferase family gene from soybean named GmGSTU23. Expression pattern analysis revealed that GmGSTU23 was predominantly expressed in the roots and flowers and exhibited a concentration-time-specific pattern in response to salt stress. Transgenic lines were generated and subjected to phenotypic characterization under salt stress. The transgenic lines exhibited increased salt tolerance, root length, and fresh weight compared to the wild type. Antioxidant enzyme activity and malondialdehyde content were subsequently measured, and the data revealed no significant differences between the transgenic and wild-type plants in the absence of salt stress. However, under salt stress, the wild-type plants exhibited significantly lower activities of SOD, POD, and CAT than the three transgenic lines, whereas the activity of APX and the content of MDA showed the opposite trend. We identified changes in glutathione pools and associated enzyme activity to gain insights into the underlying mechanisms of the observed phenotypic differences. Notably, under salt stress, the transgenic Arabidopsis's GST activity, GR activity, and GSH content were significantly higher than those of the wild type. In summary, our findings suggest that GmGSTU23 mediates the scavenging of reactive oxygen species and glutathione by enhancing the activity of glutathione transferase, thereby conferring enhanced tolerance to salt stress in plants.