文献类型: 外文期刊
作者: Fan, Guiyan 1 ; Duan, Shaoguang 1 ; Yang, Yuting 1 ; Duan, Yanfeng 1 ; Jian, Yinqiao 1 ; Hu, Jun 1 ; Liu, Zhiyuan 1 ; Guo, Yang-dong 2 ; Jin, Liping 1 ; Xu, Jianfei 1 ; Li, Guangcun 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Key Lab Biol & Genet Improvement Tuber & Root Crop, Beijing 100081, Peoples R China
2.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China
3.Shijiazhuang Univ, Shijiazhuang 050035, Hebei, Peoples R China
关键词: BSA-seq; Eye depth; Peroxidase; Potato tuber; Quantitative trait loci
期刊名称:HORTICULTURAL PLANT JOURNAL ( 影响因子:6.2; 五年影响因子:6.1 )
ISSN: 2095-9885
年卷期: 2025 年 11 卷 3 期
页码:
收录情况: SCI
摘要: Eye depth is an important agronomic trait affecting tubers' appearance, quality, and processing suitability. Hence, cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding. In this study, based on the primary mapping of the tuber eye-depth locus using a small primary-segregating population, a large secondary-segregating population with 2 100 individuals was used to map the eye-depth locus further. A major quantitative trait locus for eye-depth on chromosome 10 was identified (designated qEyd10.1) using BSAseq and traditional QTL mapping methods. The qEyd10.1 could explain 55.0% of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis. To predict candidate genes, tissue sectioning and RNA-seq of the specific tuber tissues were performed. Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates. These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms.
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