Magnetic Relaxation Switching Immunoassay Based on Hydrogen Peroxide-Mediated Assembly of Ag@Au-Fe3O4 Nanoprobe for Detection of Aflatoxin B1
文献类型： 外文期刊
作者： Dong, Yongzhen 1 ; Wen, Cong-ying 2 ; She, Yongxin 3 ; Zhang, Yu 2 ; Chen, Yiping 1 ; Zeng, Jingbin 2 ;
作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
2.China Univ Petr East China, Coll Sci, Qingdao 266580, Peoples R China
3.Chinese Acad Agr Sci, Key Lab Agroprod Qual & Safety MOA, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
关键词： aflatoxin B1; Ag@Au-Fe; O-3; (4)-MRS immunosensor; dumbbell-like Au-Fe; O-3; (4) nanoparticles; hydrogen peroxide; magnetic relaxation switching
期刊名称：SMALL （ 影响因子：13.281； 五年影响因子：12.463 ）
ISSN： 1613-6810
年卷期：
页码：
收录情况： SCI
摘要： Magnetic relaxation switching (MRS) sensors have shown great potential in food safety monitoring due to their high signal-to-noise ratio and simplicity, but they often suffer from insufficient sensitivity and stability due to the lack of excellent magnetic nanoprobes. Herein, dumbbell-like Au-Fe3O4 nanoparticles are designed as magnetic nanoprobes for developing an aflatoxin B1-MRS immunosensor. The Fe3O4 portion in the Au-Fe3O4 nanoparticles functions as the magnetic probe to provide transverse relaxation signals, while the Au segments serve as a bridge to grow Ag shell and assemble the Au-Fe3O4 nanoparticles, thus modulating transverse relaxation time of surrounding water molecular. The formation of Ag@Au-Fe3O4 is triggered by hydrogen peroxide. After degraded by horseradish peroxidase, hydrogen peroxide reduces Ag+ to Ag nanoparticles which assemble dispersed Au-Fe3O4 to aggregated Ag@Au-Fe3O4, thus dramatically improving the sensitivity of traditional MRS sensor. Combined with competitive immunoreaction, this Ag@Au-Fe3O4-MRS immunosensor can detect aflatoxin B1 with a high sensitivity (3.81 pg mL(-1)), which improved about 21 folds and 9 folds than those of enzyme-linked immunosorbent assay and high-performance liquid chromatography (HPLC), respectively. The good consistency with HPLC in real samples detection indicates the good accuracy of this immunosensor. This Ag@Au-Fe3O4-MRS immunosensor offers an attractive tool for detection of harmful substances.
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