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SsPsaH, a H subunit of the photosystem I reaction center of Suaeda salsa, confers the capacity of osmotic adjustment in tobacco

文献类型： 外文期刊

作者： Hao, Xiaoyan 1 ; Li, Jianping 1 ; Gao, Shengqi 1 ; Tuerxun, Zumuremu 1 ; Chang, Xiaochun 1 ; Hu, Wenran 1 ; Chen, Guo; 1 ;

作者机构： 1.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Urumqi 830091, Peoples R China

2.Xinjiang Acad Agr Sci, Xinjiang Key Lab Crop Biotechnol, Urumqi 830091, Peoples R China

关键词： Photosystem I; The H subunit of the Photosystem I reaction center(PsaH); Suaeda salsa; Stress tolerance

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN： 1976-9571

年卷期：

页码：

收录情况： SCI

摘要： Background Abiotic stress effects agricultural production, so research on improving stress tolerance of crop is important. Suaeda salsa is a halophyte with high salt and drought tolerance and ability to desalinate saline soil and improve soil quality. Objective To discover and utilize of salt and drought tolerance-related genes, we further investigated the mechanisms of salt and drought tolerance. Methods Through screening a salt treated Suaeda salsa cDNA library and further cloning a H subunit of the photosystem I reaction center SsPsaH cDNA, and then the protein domain and phylogenetic analyses of PSI genes was conducted with the NCBI Blast, DNAMAN, and MotifScan programs. The S. salsa seedlings were subjected to various stress treatments and analyze expression of SsPsaH under these treatments by real-time RT-PCR. SsPsaH expression construct was introduced into S. pombe cells by electroporation and transformed into N. tabacum plants by the leaf disc transformation method. Results A member of the H subunit of the Photosystem I reaction center (defined as SsPsaH) was obtained. The expression of SsPsaH was up-regulated by abscisic acid (ABA), salt, and drought stress treatments. Over-expressing SsPsaH in recombinant yeasts enhanced high salinity tolerance and increased tolerance to sorbitol during seed germination and seedling root development in tobacco, respectively. Some stress-related mark genes such as a LEA family gene of NtLEA, a binding protein of a drought response element of NtDREB, the ascorbate peroxidase gene (NtAPX) were also up-regulated in SsPsaH overexpressing transgenic tobacco lines. Conclusions These results show that SsPsaH may contribute to the salt and osmotic stress response of plants.

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