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Knockdown of the Meq gene in Marek's disease tumor cell line MSB1 might induce cell apoptosis and inhibit cell proliferation and invasion

文献类型： 外文期刊

作者： Zhao Chun-fang 1 ; Li Xin 1 ; Han Bo 1 ; Qu Lu-jiang 1 ; Liu Chang-jun 3 ; Song, Jiu Zhou 4 ; Yang Ning 1 ; Lian Ling 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Dept Anim Genet & Breeding, Natl Engn Lab Anim Breeding, Beijing 100193, Peoples R China

2.Anhui Sci & Technol Univ, Dept Anim Sci, Chuzhou 233100, Peoples R China

3.Chinese Acad Agr Sci, Harbin Vet Res Inst, Div Avian Infect Dis, Harbin 150001, Peoples R China

4.Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA

关键词： Marek's disease; Meq; proliferation; migration; invasion

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 11 期

页码：

收录情况： SCI

摘要： Marek's disease (MD), a highly cell-associated and contagious disease of chickens caused by Marek's disease virus (MDV) can result in neural lesions, immunosuppression and neoplasia in chicken. The Meq gene is an important oncogene in the MDV genome, and it is expressed highly in MD tumor tissues and MD T-lymphoblastoid cell lines. An experiment was conducted to elucidate the role of Meq in MD tumor transformation. RNA interference technology was used to block its expression, and then analyzed the biological effects of Meq knockdown on the MD tumor cell line MSB1. A small interfering RNA with an interference efficiency of 70% (P<0.01) was transfected into MSB1 cells to knock down the expression of Meq gene. The cell proliferation, cycle and apoptosis were detected post-Meq knockdown. The results showed that MSB1 cell proliferation was downregulated remarkably at 48 h (P<0.01), 60 h (P<0.05) and 72 h (P<0.01) post-Meq knockdown. The cell cycle was unaffected (P>0.05). B-cell lymphoma 2 gene (BCL2) was anti-apoptotic and caspase-6 was the effector in the apoptosis pathway. The activity of caspase-6 was upregulated (P<0.05) significantly and BCL2 gene expression was downregulated (P<0.05) significantly post-Meq knockdown, suggesting cell apoptosis might be induced. MSB1 cell migration did not exhibit any obvious change (P>0.05) post-Meq knockdown, but the expression of two genes (matrix metalloproteinase 2 (MMP2) and MMP9) that are correlated closely to cell invasion was downregulated (P<0.05) remarkably post-Meq knockdown. The Meq knockdown might affect the main features of tumorous cells, including proliferation, apoptosis, and invasion, suggesting that the Meq gene might play a crucial role in interfering with lymphomatous cell transformation.

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